Synthesis and characterization of a new interpenetrated poly(2-hydroxyethylmethacrylate)-gelatin composite polymer.
Synthesis and characterization of a new interpenetrated poly(2-hydroxyethylmethacrylate)-gelatin composite polymer.
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DOI：
10.1016/0142-9612(96)89769-9
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发表时间：
1996
期刊：
影响因子：
14
通讯作者：
M. Santin;Samuel J. Huang;Salvatore Iannace;Luigi Ambrosio;Luigi Nicolais;Gianfranco Peluso
中科院分区：
文献类型：
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作者：
M. Santin;Samuel J. Huang;Salvatore Iannace;Luigi Ambrosio;Luigi Nicolais;Gianfranco Peluso
聚（甲基丙烯酸 2-羟乙酯）[聚（HEMA）] 是一种广泛使用的生物材料，其表面不允许细胞粘附和生长，限制了其在细胞凝聚力有害的生物医学应用中的使用。我们使用顺序互穿聚合物网络技术制备了聚（HEMA）-明胶复合水凝胶。将该材料与聚(HEMA)冻干海绵在形态、力学性能和生物相容性方面进行了比较。此外，体内生物相容性实验强调了聚（HEMA）-明胶互穿聚合物网络表面上细胞相互作用的发生，而当未修饰的聚（HEMA）水凝胶植入同一宿主生物体时通常不存在这种相互作用。这些测试还表明，在短期（7 天）植入过程中，聚（HEMA）明胶样本从表面到主体的明胶逐渐降解。最后，体外测试证实了这种复合材料作为细胞支架的能力得到了改善。
Poly(2-hydroxyethylmethacrylate) [poly(HEMA)] is a widely used biomaterial which does not allow cell adhesion and growth on its surface, limiting its use in biomedical applications in which cell cohesion is detrimental. We have prepared a poly(HEMA)-gelatin composite hydrogel using a sequential interpenetrating polymer network technique. The properties of this material were compared with poly(HEMA) freeze-dried sponges in terms of morphology, mechanical properties and biocompatibility. Moreover, in vivo biocompatibility experiments highlighted the occurrence of cellular interactions on the surface of the poly(HEMA)-gelatin interpenetrating polymer network, which are usually absent when unmodified poly(HEMA) hydrogels are implanted in the same host organism. These tests also showed a progressive gelatin degradation from the surface to the bulk of the poly(HEMA)-gelatin specimens during short-term (7 d) implantation. Finally, in vitro tests confirmed an improved ability of this composite to scaffold for the cells.