Evidence for Estrogen-Dependent Uterine Serpin (SERPINA14) Expression During Estrus in the Bovine Endometrial Glandular Epithelium and Lumen1
Evidence for Estrogen-Dependent Uterine Serpin (SERPINA14) Expression During Estrus in the Bovine Endometrial Glandular Epithelium and Lumen1
复制标题
DOI：
10.1095/biolreprod.108.075184
复制
发表时间：
2009-10
期刊：
影响因子：
--
通讯作者：
S. Ulbrich;T. Frohlich;Katy Schulke;Eva Englberger;N. Waldschmitt;G. Arnold;H. Reichenbach;M. Reichenbach;E. Wolf;H. Meyer;S. Bauersachs
中科院分区：
文献类型：
--
作者：
S. Ulbrich;T. Frohlich;Katy Schulke;Eva Englberger;N. Waldschmitt;G. Arnold;H. Reichenbach;M. Reichenbach;E. Wolf;H. Meyer;S. Bauersachs
子宫分泌物对胚胎发育发生的环境有主要影响。子宫蛇形蛋白（SERPINA14，以前称为UTMP）在反刍动物怀孕期间的子宫中被发现最多。虽然目前认为黄体酮是SERPINA14表达的主要调节因子，但我们最近对发情周期牛子宫内膜转录组变化的研究意外地发现发情期SERPINA14 mRNA水平显著上调。本研究描述了SERPINA14基因的全长mRNA序列、基因组组织和假定的启动子元件。采用实时RT-PCR技术，在发情周期和妊娠早期的几个时间点，定量检测环间子宫内膜SERPINA14 mRNA的丰度。在发情期发现最高水平，随后急剧下降，在黄体期适度表达。在第18天，与对照组相比，妊娠子宫内膜的转录物水平更高。发情时，免疫反应蛋白定位于深部腺上皮，Western blotting同时显示子宫冲洗液呈52-kDa形态。经雌二醇-17 β和孕酮刺激后，体外子宫内膜腺细胞SERPINA14 mRNA表达显著上调，而经干扰素-tau治疗后则无显著上调。我们的结果清楚地表明，SERPINA14在发情期牛子宫内膜中明显出现。假设子宫内膜为配子，尤其是精子的传递提供了一个准备良好的子宫内膜环境。
Uterine secretions have a dominant impact on the environment in which embryo development takes place. The uterine serpins (SERPINA14, previously known as UTMP) are found most abundantly during pregnancy in the uterus of ruminants. Although progesterone is currently assumed to be the major regulator of SERPINA14 expression, our recent study of transcriptome changes in bovine endometrium during the estrous cycle unexpectedly detected a marked upregulation of SERPINA14 mRNA levels at estrus. The present study describes the full-length mRNA sequence, genomic organization, and putative promoter elements of the SERPINA14 gene. The SERPINA14 mRNA abundance was quantified by real-time RT-PCR in intercaruncular endometrium at several time points during the estrous cycle and early pregnancy. Highest levels were found at estrus, followed by a dramatic decrease and a moderate expression during the luteal phase. Transcript levels were higher in pregnant endometrium compared with controls at Day 18. At estrus, immunoreactive protein was localized in deep glandular epithelium, and Western blotting concomitantly showed the 52-kDa form in uterine flushings. SERPINA14 mRNA was significantly upregulated in glandular endometrial cells in vitro after stimulation with estradiol-17beta and progesterone, but not after interferon-tau treatment. Our results clearly demonstrate that SERPINA14 appears distinctly in bovine endometrium during the estrus phase. A supporting role toward providing a well-prepared endometrial environment for passing gametes, especially sperm, is assumed.