First Report of Potato spindle tuber viroid in Cape Gooseberry (Physalis peruviana) from Turkey and Germany.
First Report of Potato spindle tuber viroid in Cape Gooseberry (Physalis peruviana) from Turkey and Germany.
复制标题
来自土耳其和德国的醋栗（Physalis peruviana）中马铃薯纺锤块茎类病毒的首次报告。
DOI：
10.1094/pdis-93-3-0316a
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发表时间：
2009
期刊：
影响因子：
4.5
通讯作者：
E. Meekes
中科院分区：
文献类型：
--
作者：
J. Verhoeven;M. Botermans;J. Roenhorst;J. Westerhof;E. Meekes
自从最近在无性繁殖的观赏植物物种中发现了马铃薯纺锤块茎类病毒（PSTVd）以来，许多种植者要求对其母株进行这种类病毒检测。2007年12月，一位来自土耳其的种植者提交了醋栗（Physalis peruviana）插枝进行PSTVd检测。Boonham等人(1)通过实时反转录(RT)-PCR进行的初步检测表明，四个样本中存在墨西哥木瓜类病毒、PSTVd或番茄绿矮化类病毒。为了鉴定存在的类病毒，将从这些样本中分离的RNA用于RT-PCR(2)，并从每个样本中扩增出三种类病毒的预期全基因组大小的产物。对其中一个PCR产物进行测序(GenBank登录号：；EU862230)和对357 nt序列的分析表明，它与属于所谓的“大洋洲”毒株的PSTVd序列最相关(3)，与GenBank登录号为99.7%的同源性。AY962324。因此，该类病毒被鉴定为PSTVd。该PSTVd基因型的致病性在机械接种番茄幼苗4周后得到证实。金银花表现出了预期的类病毒症状，如萎黄和发育迟缓，并通过RT-PCR证实了类病毒在这些植物中的存在(2)。2008年3月，通过RT-PCR(2)和PCR产物测序(GenBank登录号：EU862231)和PSTVd在德国佩鲁亚纳（P. peruviana）幼苗中得到鉴定。德国分离物与土耳其分离物仅在三个核苷酸位置上不同。幼苗中PSTVd的鉴定表明，种子是感染源，而在土耳其的PSTVd感染插枝的情况下，感染来自被感染的母植物。据我们所知，这是秘鲁紫花葡萄属PSTVd的首次报道。虽然受感染的秘鲁假葡萄植株没有表现出症状，但它们可能成为马铃薯和番茄等作物的接种源，这些作物可能遭受严重损害。参考文献：(1)N. Boonham等。j .性研究。方法116:139,2004。(2) A. M. Shamloul等。可以。植物病理学报，19(9),1997。(3)解析：J. Verhoeven等。欧元。植物学报，2009,31(2):481 - 481。(4)解析：J. Verhoeven等。植物病理学杂志，2008。
Since the recent identification of Potato spindle tuber viroid (PSTVd) in vegetatively propagated ornamental plant species (4), many growers have asked to have their mother plants tested for this viroid. In December of 2007, a grower from Turkey submitted cuttings of cape gooseberry (Physalis peruviana) to be tested for PSTVd. Initial testing by real-time reverse transcription (RT)-PCR according to Boonham et al. (1) indicated the presence of either Mexican papita viroid, PSTVd, or Tomato chlorotic dwarf viroid in four samples. To identify the viroid(s) present, isolated RNA from these samples was used for RT-PCR (2), and products of the expected full genome size for the three viroids were amplified from each sample. One of the PCR products was sequenced (GenBank Accession No. EU862230) and analysis of the 357 nt sequence indicated it was most related to PSTVd sequences belonging to the so-called 'Oceanian' strain of the viroid (3), with 99.7% identity to GenBank Accession No. AY962324. Therefore, the viroid was identified as PSTVd. Pathogenicity of this PSTVd genotype was demonstrated when 4 weeks after mechanical inoculation with sap extracts seedlings of tomato cv. Money-maker showed the expected viroid symptoms of chlorosis and stunting, and the presence of the viroid in these plants was confirmed by RT-PCR (2). In March of 2008, by use of RT-PCR (2) and sequencing of the PCR product (GenBank Accession No. EU862231), PSTVd was identified in young seedlings of P. peruviana from a German grower. The German isolate differed at only three nucleotide positions from the Turkish isolate. The identification of PSTVd in young seedlings indicates that seeds had been source of infection, whereas in the case of the PSTVd infected cuttings from Turkey, the infection originated from infected mother plants. To our knowledge, these are the first reports of PSTVd in P. peruviana. Although infected P. peruviana plants did not show symptoms, they might act as sources of inoculum for crops like potato and tomato, which may suffer serious damage. References: (1) N. Boonham et al. J. Virol. Methods 116:139, 2004. (2) A. M. Shamloul et al. Can. J. Plant Pathol. 19:89, 1997. (3) J. Th. J. Verhoeven et al. Eur. J. Plant Pathol. 110:823, 2004. (4) J. Th. J. Verhoeven et al. Plant Pathol. 57:399, 2008.