Histidine dipeptides HPLC-based test for the detection of mammalian origin proteins in feeds for ruminants
Histidine dipeptides HPLC-based test for the detection of mammalian origin proteins in feeds for ruminants
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DOI：
10.1016/j.meatsci.2003.10.008
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发表时间：
2004-06-01
期刊：
影响因子：
7.1
通讯作者：
Toldrá, F
中科院分区：
文献类型：
--
作者：
Aristoy, MC;Toldrá, F
目前的工作重点是利用相对数量的肌肽和安丝氨酸来检测反刍动物饲料中哺乳动物来源的蛋白质的存在。该方法廉价、简单，可用于饲料的快速筛选。基于对肌肽和/或鹅丝氨酸二肽的阳离子交换高效液相测定，可以很容易地检测到添加到饲料中的低至0.5%的动物蛋白。此外，这些二肽的摩尔比被发现是动物物种的特征，可以用来区分最终添加到饲料中的哺乳动物和非哺乳动物物种。肌氨酸/安丝氨酸摩尔比高于0.3的饲料被强烈怀疑含有违禁哺乳动物蛋白质。这些可疑的馈送稍后可能会通过更具体的技术进行确认。这种方法无法确定含有不同物种混合动物蛋白的饲料的确切来源，即使这种方法能够检测到其来自哺乳动物的来源。(C)2003爱思唯尔有限公司。保留所有权利。
The present work is focused oil the use of the relative amounts of carnosine and anserine for detecting the presence of proteins of mammalian origin in feeds for ruminants. The methodology is cheap and simple and may serve for the rapid screening of feeds. Animal protein additions to feeds as low as 0.5% can be easily detected based on the cation exchange HPLC determination of the carnosine and/or anserine dipeptides. Furthermore, the molar ratio of these dipeptides was found to be characteristic of the animal species and could be used to discriminate between mammalian and non-mammalian species eventually added to feeds. Feeds having carnosine/anserine molar ratios higher than 0.3 were strongly suspected of containing banned mammalian proteins. Those suspicious feeds might be later confirmed by more specific techniques. The exact Source of feeds containing Mixtures of animal proteins from different species could not be identified by this method even though the method was able to detect its mammalian origin. (C) 2003 Elsevier Ltd. All rights reserved.