Novel herpes simplex virus type 1 glycoproteins identified by antiserum against a synthetic oligopeptide from the predicted product of gene US4.
Novel herpes simplex virus type 1 glycoproteins identified by antiserum against a synthetic oligopeptide from the predicted product of gene US4.
复制标题
通过针对基因 US4 预测产物的合成寡肽的抗血清鉴定出新型单纯疱疹病毒 1 型糖蛋白。
DOI：
--
复制
发表时间：
1986
影响因子：
3.8
通讯作者：
D. McGeoch
中科院分区：
文献类型：
--
作者：
M. Frame;H. Marsden;D. McGeoch
单纯疱疹病毒1型（HSV-1）基因US 4编码一种分子量为25237的蛋白质，其性质表明它是一种膜结合蛋白。我们已经研究了这种蛋白质，通过提高抗血清的合成寡肽对应于一段氨基酸从内部亲水区域的预测序列。该抗血清免疫沉淀三种表观分子量为1000的糖蛋白。重量37 000，48 000和56 000从HSV-1感染的细胞提取物。这些物种也特异性地从纯化的病毒粒子免疫沉淀。US 4基因的体外翻译产物具有表观分子量。重量35 000人。HSV-1和HSV-2基因组的短独特区域的序列比较，结合HSV-2的糖蛋白G（gG）的已发表的作图数据，得出结论：HSV-1的基因US 4的产物是gG的等价物。
Gene US4 of herpes simplex virus type 1 (HSV-1) has been predicted, from DNA sequence analysis, to encode a protein of molecular weight 25237 and its properties suggest it to be a membrane-associated protein. We have investigated this protein by raising antiserum to a synthetic oligopeptide corresponding to a stretch of amino acids from an internal hydrophilic region of the predicted sequence. This antiserum immunoprecipitates three glycoprotein species of apparent mol. wt. 37 000, 48 000 and 56 000 from extracts of cells infected with HSV-1. These species are also specifically immunoprecipitated from purified virions. The in vitro translation product of gene US4 has an apparent mol. wt. of 35 000. Sequence comparisons of the short unique regions of the HSV-1 and HSV-2 genomes, in combination with published mapping data for glycoprotein G (gG) of HSV-2, has led to the conclusion that the product of gene US4 of HSV-1 is the equivalent of gG.