A taxonomic study of Eudorina unicocca (Volvocaceae, Chlorophyceae) and related species, based on morphology and molecular phylogeny
A taxonomic study of Eudorina unicocca (Volvocaceae, Chlorophyceae) and related species, based on morphology and molecular phylogeny
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DOI：
10.1080/09670260701763484
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发表时间：
2008-08
影响因子：
2.4
通讯作者：
T. Yamada;K. Miyaji;H. Nozaki
中科院分区：
文献类型：
--
作者：
T. Yamada;K. Miyaji;H. Nozaki
群体性的volvocacea藻类进行两种类型的有性生殖：同配生殖和异配生殖/有精子包的卵配生殖。这种差异是团藻科中一个重要的属的诊断。虽然Yamagishiella与异花型的Eudorina属在同花型有性生殖方面存在差异，但两属的营养形态和无性生殖特征是不可区分的，特别是Eudorina unicocca G. M. Smith和单裂山木氏菌Yamagishiella unicocca（Rayburn et Starr）Nozaki。我们重新研究了E. unicocca和相关种，使用多株E. unicocca和Y. unicocca和分子系统发育分析。来自日本两个湖泊的菌株，产生不动孢子，并且是完全无性的，可以归属于E。unicocca或Y. unicocca，基于传统的形态学诊断。然而，新的形态学诊断（细胞表面收缩泡的分布和数量的差异）和分子系统发育分析表明，所有的E。unicocca。此外，还发现E.根据菌落凝胶状基质中单个细胞鞘的存在或不存在，可以将unicocca分为两个种，这可以用亚甲蓝染色观察到。这两个物种，E. peripheralis（Goldstein）T. K.山田nov.（= E.乌尼科卡湾peripherialis Goldstein）和E. unicocca（包括日本的不动孢子菌株），形成了两个强大的单系群，基于大RuBisCO亚基的叶绿体基因序列和核核糖体DNA的内部转录间隔区。
Colonial volvocacean algae engage in two types of sexual reproduction: isogamy and anisogamy/oogamy with sperm packets. This difference is an important generic diagnosis within the Volvocaceae. Although Yamagishiella differs from the anisogamous genus Eudorina in its isogamous sexual reproduction, the vegetative morphology and asexual reproduction characteristics of the two genera are indistinguishable, especially between Eudorina unicocca G. M. Smith and Yamagishiella unicocca (Rayburn et Starr) Nozaki. We re-examined morphological characteristics of E. unicocca and related species, using multiple strains of E. unicocca and Y. unicocca and molecular phylogenetic analyses. Strains from two Japanese lakes, which produced aplanospores and were solely asexual, could be assigned to either E. unicocca or Y. unicocca, based on traditional morphological diagnoses. However, a new morphological diagnosis (the difference in the distribution and number of contractile vacuoles on the cell surface) and molecular phylogenetic analyses demonstrated that all were E. unicocca. Furthermore, E. unicocca can be divided into two species on the basis of the presence or absence of individual cellular sheaths in the colonial gelatinous matrix, which are observable with methylene blue staining. These two species, E. peripheralis (Goldstein) T. K. Yamada stat. nov. (= E. unicocca var. peripherialis Goldstein) and E. unicocca (including the Japanese aplanosporic strains), formed two robust monophyletic groups, based on chloroplast gene sequences for the large RuBisCO subunit and internal transcribed spacer regions of nuclear ribosomal DNA.