Highly sensitive and selective detection of dopamine using one-pot synthesized highly photoluminescent silicon nanoparticles.
Highly sensitive and selective detection of dopamine using one-pot synthesized highly photoluminescent silicon nanoparticles.
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DOI：
10.1021/ac504520g
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发表时间：
2015-02
影响因子：
7.4
通讯作者：
Xiaodong Zhang;Xiaokai Chen;Siqi Kai;Hong-Yin Wang;Jingjing Yang;Fu‐Gen Wu;Zhan Chen
中科院分区：
文献类型：
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作者：
Xiaodong Zhang;Xiaokai Chen;Siqi Kai;Hong-Yin Wang;Jingjing Yang;Fu‐Gen Wu;Zhan Chen
报道了一种简单高效的水溶性硅纳米颗粒(SiNPs)检测多巴胺(DA)的方法。采用一锅法合成了量子产率高达23.6%的SiNPs。测试了各种分子对合成的SiNPs的荧光猝灭能力，发现只有DA分子能够有效地猝灭这些SiNPs的荧光。因此，这种猝灭效应可用于选择性地检测DA。所有其他被测试的分子对多巴胺的检测几乎没有干扰，包括抗坏血酸，它普遍存在于细胞中，可能会影响多巴胺的检测。猝灭和未猝灭情况下的荧光强度差与未猝灭情况下的荧光强度之比(ΔI/I)与DA分析物浓度在0.005~10.0μM范围内呈线性关系，检出限为0.3 nm(S/N=3)。据我们所知，这是迄今为止报告的DA检测的最低限度。荧光猝灭的机理可归结为SiNPs通过Förster共振能量转移将能量传递给被氧化的多巴胺分子。已报道的合成SiNP的方法非常简单和廉价，使得利用SiNPs的上述灵敏和选择性的DA检测方法具有实际应用价值。
A simple and highly efficient method for dopamine (DA) detection using water-soluble silicon nanoparticles (SiNPs) was reported. The SiNPs with a high quantum yield of 23.6% were synthesized by using a one-pot microwave-assisted method. The fluorescence quenching capability of a variety of molecules on the synthesized SiNPs has been tested; only DA molecules were found to be able to quench the fluorescence of these SiNPs effectively. Therefore, such a quenching effect can be used to selectively detect DA. All other molecules tested have little interference with the dopamine detection, including ascorbic acid, which commonly exists in cells and can possibly affect the dopamine detection. The ratio of the fluorescence intensity difference between the quenched and unquenched cases versus the fluorescence intensity without quenching (ΔI/I) was observed to be linearly proportional to the DA analyte concentration in the range from 0.005 to 10.0 μM, with a detection limit of 0.3 nM (S/N = 3). To the best of our knowledge, this is the lowest limit for DA detection reported so far. The mechanism of fluorescence quenching is attributed to the energy transfer from the SiNPs to the oxidized dopamine molecules through Förster resonance energy transfer. The reported method of SiNP synthesis is very simple and cheap, making the above sensitive and selective DA detection approach using SiNPs practical for many applications.