RNA-seq analysis reveals new candidate genes for drip loss in a Pietrain x Duroc x Landrace x Yorkshire population
RNA-seq analysis reveals new candidate genes for drip loss in a Pietrain x Duroc x Landrace x Yorkshire population
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RNA-seq分析揭示了皮特兰×杜洛克×长白×约克夏种群中滴水损失的新候选基因
DOI：
10.1111/age.12401
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发表时间：
2016
期刊：
影响因子：
2.4
通讯作者：
Liu Honglin
中科院分区：
文献类型：
--
作者：
Li Bojiang;Liu Kaiqing;Weng Qiannan;Li Pinghua;Wei Wei;Li Qifa;Chen Jie;Huang Ruihua;Wu Wangjun;Liu Honglin
滴水损失是最重要的肉质性状之一，具有遗传力低的特点。到目前为止，影响滴水损失性状的遗传因素还没有清楚地阐明。这项研究的目的是确定影响滴漏的关键候选基因。首先，我们建立了皮特兰×杜洛克×长白×约克郡商品猪群体，并获得了滴水损失性状的表型值。此外，我们还从水滴损失最高(H组)和最低(L组)的背最长肌样本中构建了两个文库，并利用RNAseq技术鉴定了这两种极端表型之间的差异表达基因(Deg)。在H组和L组文库中共检测到25883个基因，没有一个基因在一个文库中特异表达。基因表达水平的比较分析发现，与L组相比，H组有150个基因表达差异，其中有127个基因上调，23个基因下调。此外，还检测到68个与63°重叠的滴水损失数量性状基因座(QTL)，这些QTL主要分布在第1、2、5和6染色体上。有趣的是，与肌肉收缩和脂肪沉积有关的三联素(TRDN)基因和与肌肉生长有关的肌肉生长抑制素(MSTN)基因被定位在两个以上的滴水损失QTL上，这表明这两个基因都是导致滴水损失的关键候选基因。
Drip loss, one of the most important meat quality traits, is characterized by low heritability. To date, the genetic factors affecting the drip loss trait have not been clearly elucidated. The objective of this study was to identify critical candidate genes affecting drip loss. First, we generated a Pietrain × Duroc × Landrace × Yorkshire commercial pig population and obtained phenotypic values for the drip loss trait. Furthermore, we constructed two RNA libraries from pooled samples of longissimus dorsi muscles with the highest (H group) and lowest (L group) drip loss and identified the differentially expressed genes (DEGs) between these extreme phenotypes using RNA‐seq technology. In total, 25 883 genes were detected in the H and L group libraries, and none was specifically expressed in only one library. Comparative analysis of gene expression levels found that 150 genes were differentially expressed, of which 127 were upregulated and 23 were downregulated in the H group relative to the L group. In addition, 68 drip loss quantitative trait loci (QTL) overlapping with 63 DEGs were identified, and these QTL were distributed mainly on chromosomes 1, 2, 5 and 6. Interestingly, thetriadin(TRDN) gene, which is involved in muscle contraction and fat deposition, and themyostatin(MSTN) gene, which has a role in muscle growth, were localized to more than two drip loss QTL, suggesting that both are critical candidate genes responsible for drip loss.