Submerged vascular anastomosis. A technique for vascular suturing in experimental microsurgery.
Submerged vascular anastomosis. A technique for vascular suturing in experimental microsurgery.
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DOI：
10.1590/acb360807
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发表时间：
2021
影响因子：
1.1
通讯作者：
Fernandes MS
中科院分区：
文献类型：
--
作者：
Menezes Neto BF;Oliveira Neto FV;Secanho MS;Carvalho LB;Moragas WR;Fernandes MS
旨在评价医学生和住院医师培训期间在实验室中使用生理盐水（0.9% NaCl）浸没显微外科吻合缝合区域的影响。选择Wistar大鼠（n = 10），将两条股动脉切片，并在光学放大下端对端闭合。将其随机分组，使一侧在盐水浸没下进行冲洗，对侧在手术过程中保持干燥。评价手术时间以及手术后30 min和72 h内的通畅率。6只雄性Wistar大鼠在手术麻醉过程中存活，平均初始体重为243.3 g，平均动脉直径为0.86 mm，浸没技术的平均时间为15.67 min，干燥技术的平均时间为20.50 min（p = 0.03）。浸没组和干燥组的失败率分别为17%和50%（p = 0.62）。浸没式微血管缝合不会影响血管的通畅性或增加吻合时间。因此，这是外科医生可以根据其技术偏好应用的策略。
To evaluate the impact of submersion of the microsurgical anastomosis suture area using saline (0.9% NaCl) in an experimental laboratory during the training of medical students and resident physicians. Wistar rats (n = 10) were selected to have the two femoral arteries sectioned and anastomosed end-to-end under optical magnification. They were randomly divided, so that on one side suturing was performed under submersion with saline, and the contralateral side was kept dry during the procedure. The surgical times, as well as the patency within 30 min and 72 h of the procedure, were evaluated. Six male Wistar rats survived the surgical anesthetic procedure, with the average initial weight of 243.3 g and the average artery diameter of 0.86 mm, with average time of 15.67 min for the submerged technique and 20.50 min for the dry technique (p = 0.03). The failure rates were 17 and 50% for the submerged group and the dry one, respectively (p = 0.62). Submerged microvascular suture does not compromise the patency of the vessel or increase the time of anastomosis. Therefore, it is a strategy that can be applied by the surgeon according to his/her technical preferences.