Nonsurgical embryo transfer device compared with surgery for embryo transfer in mice.
Nonsurgical embryo transfer device compared with surgery for embryo transfer in mice.
复制标题
DOI：
--
复制
发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Kendra H Steele;J. Hester;Barbara J. Stone;K. Carrico;B. Spear;A. Fath-Goodin
中科院分区：
文献类型：
--
作者：
Kendra H Steele;J. Hester;Barbara J. Stone;K. Carrico;B. Spear;A. Fath-Goodin
先前已经描述了使用鼠非手术胚胎移植（NSET）装置来移植胚泡、桑椹胚、DNA显微注射胚胎和含胚胎干细胞的胚胎以产生遗传修饰小鼠。然而，NSET器械和传统手术方法的生理效应尚未直接比较。在这里，我们使用心电图和粪便皮质酮水平监测假孕小鼠，只接受麻醉，NSET程序有或没有麻醉，或手术。这些程序在不使用实际胚胎的情况下进行，以关注程序本身的影响，而不是由于胚胎沉积而引起的任何生理影响。与手术和麻醉相比，NSET程序与心律波动较小和应激生物标志物粪便皮质酮水平较低有关。这些结果表明，使用NSET装置避免了这些生理扰动以及手术的其他缺点（例如，术后疼痛和需要术后镇痛），因此提供了对现有小鼠胚胎移植程序的有价值的改进。
The use of a murine nonsurgical embryo transfer (NSET) device had been described previously for the transfer of blastocysts, morulae, DNA-microinjected embryos, and embryonic stem cell-containing embryos to create genetically modified mice. However, physiologic effects of the NSET device and traditional surgical methods had not been compared directly. Here we used electrocardiography and fecal corticosterone levels to monitor pseudopregnant mice that underwent anesthesia only, the NSET procedure with or without anesthesia, or surgery. These procedures were performed without the use of actual embryos, to focus on effects of the procedures themselves rather than on any physiologic effects due to the deposition of embryos. As compared with surgery and anesthesia, the NSET procedure was associated with less fluctuation in cardiac rhythm and lower levels of the stress biomarker fecal corticosterone. These results indicate that use of the NSET device avoids these physi- ological perturbations as well as other disadvantages of surgery (for example, postoperative pain and need for postoperative analgesia) and therefore provides a valuable refinement of existing mouse embryo transfer procedures.