Mesenchymal progenitor cells derived from chorionic villi of human placenta for cartilage tissue engineering.
Mesenchymal progenitor cells derived from chorionic villi of human placenta for cartilage tissue engineering.
复制标题
DOI：
10.1016/j.bbrc.2005.12.091
复制
发表时间：
2006-02
影响因子：
3.1
通讯作者：
Xiaohong Zhang;A. Mitsuru;K. Igura;Kenji Takahashi;S. Ichinose;Satoru Yamaguchi;Tsuneo A. Takahashi
中科院分区：
文献类型：
--
作者：
Xiaohong Zhang;A. Mitsuru;K. Igura;Kenji Takahashi;S. Ichinose;Satoru Yamaguchi;Tsuneo A. Takahashi
人类间充质干细胞目前正在被广泛研究，因为它们具有自我更新和分化为各种结缔组织的能力，这使得它们作为再生医学的细胞来源具有吸引力。在此，我们报告了分离的人胎盘来源的间充质细胞（hPDMCs），有可能分化成各种谱系，以探索使用这些细胞的软骨再生的可能性。我们首先在体外评估了hPDMCs的软骨形成，然后将hPDMCs包埋到去端胶原凝胶中，用软骨形成诱导培养基制备软骨样组织。在体内试验中，将包埋在胶原海绵中的预诱导的hPDMCs皮下植入裸鼠和具有骨软骨缺损的裸鼠中。这些体内和体外研究的结果表明，hPDMCs可以作为软骨组织工程的可能的同种异体细胞来源之一。
Human mesenchymal stem cells are currently being studied extensively because of their capability for self-renewal and differentiation to various connective tissues, which makes them attractive as cell sources for regenerative medicine. Herein we report the isolation of human placenta-derived mesenchymal cells (hPDMCs) that have the potential to differentiate into various lineages to explore the possibility of using these cells for regeneration of cartilage. We first evaluated the chondrogenesis of hPDMCs in vitro and then embedded the hPDMCs into an atelocollagen gel to make a cartilage-like tissue with chondrogenic induction media. For in vivo assay, preinduced hPDMCs embedded in collagen sponges were subcutaneously implanted into nude mice and also into nude rats with osteochondral defect. The results of these in vivo and in vitro studies suggested that hPDMCs can be one of the possible allogeneic cell sources for tissue engineering of cartilage.