The Generation and Functional Characterization of Human Microglia-Like Cells Derived from iPS and Embryonic Stem Cells.
The Generation and Functional Characterization of Human Microglia-Like Cells Derived from iPS and Embryonic Stem Cells.
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iPS 和胚胎干细胞衍生的人类小胶质细胞样细胞的生成和功能表征。
DOI：
10.1007/978-1-0716-3287-1_6
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Huang,Yu-WenAlvin
中科院分区：
文献类型：
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作者：
Lehoux,Mikael;Connolly,Kevin;Assetta,Benedetta;Huang,Yu-WenAlvin
以下协议描述了使用 StemCell Technologies 的市售试剂盒从人诱导多能干细胞 (hiPSC) 生成小胶质细胞。该协议由三个主要步骤组成：(1) 造血前体细胞的分化，(2) 小胶质细胞分化，(3) 小胶质细胞成熟。描述了表征造血前体细胞和成熟小胶质细胞的测定。
The following protocol describes the generation of microglia cells from human-induced pluripotent stem cells (hiPSCs) using commercially available kits by StemCell Technologies. This protocol consists of three major steps: (1) Differentiation of hematopoietic precursor cells, (2) Microglia differentiation, and (3) Microglia maturation. Assays are described to characterize hematopoietic precursor cells and mature microglia.