Readily Available Tissue-Engineered Vascular Grafts
Readily Available Tissue-Engineered Vascular Grafts
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DOI：
10.1126/scitranslmed.3001426
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发表时间：
2011-02-02
影响因子：
17.1
通讯作者：
Niklason, Laura E.
中科院分区：
文献类型：
--
作者：
Dahl, Shannon L. M.;Kypson, Alan P.;Niklason, Laura E.
自体或合成血管移植物通常用于血液透析或心血管疾病患者的动脉旁路。然而，一些患者要么缺乏合适的自体组织，要么不能接受合成移植物。这样的病人可以从组织工程制造的血管移植物中受益。在这里，我们工程血管移植使用人同种异体或犬平滑肌细胞生长在管状聚乙醇酸支架。用去污剂去除细胞物质以使移植物无免疫原性。人体血管移植物的机械性能与人体天然血管相似，并且移植物可以在4 ℃下长期储存。在狒狒血液透析动静脉通路模型中测试了人类工程移植物。在犬外周动脉和冠状动脉搭桥模型中测试犬移植物。移植物表现出良好的通畅性，并抵抗扩张、钙化和内膜增生。这种组织工程血管移植物可以为没有合适的自体组织的患者或不适合合成移植物的患者提供容易获得的选择。
Autologous or synthetic vascular grafts are used routinely for providing access in hemodialysis or for arterial bypass in patients with cardiovascular disease. However, some patients either lack suitable autologous tissue or cannot receive synthetic grafts. Such patients could benefit from a vascular graft produced by tissue engineering. Here, we engineer vascular grafts using human allogeneic or canine smooth muscle cells grown on a tubular polyglycolic acid scaffold. Cellular material was removed with detergents to render the grafts nonimmunogenic. Mechanical properties of the human vascular grafts were similar to native human blood vessels, and the grafts could withstand long-term storage at 4 degrees C. Human engineered grafts were tested in a baboon model of arteriovenous access for hemodialysis. Canine grafts were tested in a dog model of peripheral and coronary artery bypass. Grafts demonstrated excellent patency and resisted dilatation, calcification, and intimal hyperplasia. Such tissue-engineered vascular grafts may provide a readily available option for patients without suitable autologous tissue or for those who are not candidates for synthetic grafts.