Oral administration of Bacillus subtilis promotes homing of CD3+ T cells and IgA-secreting cells to the respiratory tract in piglets
Oral administration of Bacillus subtilis promotes homing of CD3+ T cells and IgA-secreting cells to the respiratory tract in piglets
复制标题
口服枯草芽孢杆菌可促进仔猪 CD3(+) T 细胞和 IgA 分泌细胞归巢至呼吸道。
DOI：
10.1016/j.rvsc.2021.03.006
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发表时间：
2021-03-20
影响因子：
2.4
通讯作者：
Yang, Qian
中科院分区：
文献类型：
--
作者：
Zhang, Penghao;Huang, Lulu;Yang, Qian
口服益生菌用于诱导肠道免疫反应，以防止感染。然而，口服益生菌也可能影响其他粘膜组织（如呼吸道）的免疫反应。为了研究这种可能性，我们探索了口服枯草芽孢杆菌后免疫细胞归巢呼吸系统的潜力。结果表明，B.枯草芽孢杆菌能促进肠道发育，不引起呼吸道病变。口服B后。枯草杆菌感染后，肠道IgA分泌细胞和CD 3 + T细胞数量均显著增加（P < 0.01），呼吸道IgA分泌细胞和CD 3 + T细胞数量也显著增加（P < 0.01）。此外，口服B后，气管、肺、回肠和空肠中的分泌型伊加水平显著升高。与对照组相比，枯草杆菌组的作用更明显（P < 0.05）。B治疗后，白细胞介素（IL）-1 β、IL-5、IL-6、肿瘤坏死因子-α、B细胞活化因子和IgA诱导蛋白的mRNA表达增加。在气管、肺、回肠和空肠中的枯草杆菌施用（P < 0.01）。这些数据表明，B。枯草芽孢杆菌的施用不仅调节仔猪肠中的免疫应答，而且调节仔猪呼吸道中的免疫应答。我们的工作突出了一个潜在的新策略，促进呼吸道粘膜免疫，并可能有助于设计疫苗与B。枯草杆菌作为粘膜佐剂。
Oral probiotics are used to induce immune responses in the intestines to protect against infection. However, oral probiotics may also affect immune responses in other mucosal tissues such as in the respiratory tract. To examine this possibility, we explored the potential of immunocytes to home to the respiratory system after oral administration of Bacillus subtilis. The results showed that B. subtilis could promote intestinal development and not cause pathological changes in the respiratory tract. Following the oral administration with B. subtilis, the number of IgA-secreting cells and CD3+ T cells not only significantly increased in the intestinal tracts but also in respiratory tracts (P < 0.01). Moreover, the levels of secretory IgA were significantly higher in the trachea, lungs, ileum, and jejunum after oral B. subtilis administration than in the control groups (P < 0.05). The mRNA expression of interleukin (IL)-1 beta, IL-5, IL-6, tumor necrosis factor-alpha, B cell activating factor, and IgA-inducing protein increased following B. subtilis administration (P < 0.01) in the trachea, lungs, ileum, and jejunum. These data suggest that B. subtilis administration regulates the immune response not only in the intestine but also in the respiratory tract of piglets. Our work highlights a potentially new strategy for promoting respiratory mucosal immunity and may contribute to the design of vaccines with B. subtilis as a mucosal adjuvant.