A polysaccharide-protein complex from Haemophilus influenzae type b. II. Human antibodies to its somatic components.
A polysaccharide-protein complex from Haemophilus influenzae type b. II. Human antibodies to its somatic components.
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b 型流感嗜血杆菌的多糖-蛋白质复合物。
DOI：
10.1093/infdis/144.6.521
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Smith,DH
中科院分区：
文献类型：
--
作者：
Insel,RA;Anderson,P;Loeb,MR;Smith,DH
用B型流感嗜血杆菌埃根菌株制备的多糖蛋白复合物作为检测抗原，应用酶联免疫吸附试验检测人血清抗体。使用不含去污剂的洗涤缓冲液，该测定法检测脂多糖（LPS）和非LPS体细胞抗原以及多聚核糖基核糖醇磷酸（PRP）的抗体。用含有去污剂的缓冲液，未检测到LPS抗体，而未检测到非LPS体细胞组分和PRP抗体。类似地，纯化的LPS可以仅与前一种缓冲液一起用作测试抗原。健康成人和儿童中普遍存在非LPS体细胞抗原的IgG、伊加和IgM抗体，15例因H而从脑膜炎恢复的患者中有14例的水平升高。流感病毒B型，IgG型占优势。LPS抗体普遍存在，但浓度较低，IgM是主要类型; 15例患者中有12例水平升高。
A polysaccharide-protein complexprepared fromHaemophilus influenzaetype b strain Eagan was used as test antigen in an enzyme-linked immunosorbent assay for human serum antibodies. With washing buffer that did not contain detergent, the assay detected antibody to lipopolysaccharide (LPS) and to non-LPS somatic antigensas well as to polyribosylribitolphosphate (PRP). With buffer that did contain detergent, antibodies to LPS were not detected, whereas detection of antibodies to the non-LPS somatic components and to PRP was unimpeded. Similarly, purified LPS could be used as test antigen with the former buffer only. IgG, IgA, and IgM antibodies to non-LPS somatic antigens were prevalent in healthy adults and children, and levels increased in 14 of 15 patients recovering from meningitis due toH. influenzaetype b; IgG was the predominant class. Antibodies to LPS wereprevalent but at lower concentrations, and IgM was the predominant class; levels increased in 12 of the 15 patients.