Placental alkaline phosphatase expression at the apical and basal plasma membrane in term villous trophoblasts
Placental alkaline phosphatase expression at the apical and basal plasma membrane in term villous trophoblasts
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DOI：
10.1177/002215540104900909
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发表时间：
2001-09-01
影响因子：
3.2
通讯作者：
Fuchs, R
中科院分区：
文献类型：
--
作者：
Leitner, K;Szlauer, R;Fuchs, R
使用免疫电子显微镜观察，人胎盘碱性磷酸酶（PLAP）位于合体滋养细胞的顶端和基底质膜以及足月绒毛膜绒毛中细胞滋养细胞的表面。同样，在极化滋养层来源的 BeWo 细胞中发现顶端和基底外侧 PLAP 表达。从足月胎盘分离的滋养层在分离后立即主要表现出囊泡PLAP免疫荧光染色。体外分化为合胞体后，PLAP 质膜表达上调并超过单核滋养层中观察到的表达。这些数据要求谨慎使用 PLAP 作为形态学标记来区分合体滋养层和细胞滋养层，或作为胎盘刷状缘膜的标记酶。
Human placental alkaline phosphatase (PLAP) was localized at the apical and basal plasma membrane of syncytiotrophoblasts and at the surface of cytotrophoblasts in term chorionic villi using immunoelectron microscopy. Similarly, apical and basolateral PLAP expression was found in polarized trophoblast-derived BeWo cells. Trophoblasts isolated from term placentas exhibited mainly vesicular PLAP immunofluorescence staining immediately after isolation. After in vitro differentiation into syncytia, PLAP plasma membrane expression was upregulated and exceeded that observed in mononuclear trophoblasts. These data call for caution in using PLAP as a morphological marker to differentiate syncytiotrophoblasts from cytotrophoblasts or as a marker enzyme for placental brush-border membranes.