Cells Mediating Delayed‐Type Hypersensitivity in the Lungs of Mice Infected with an Influenza A Virus
Cells Mediating Delayed‐Type Hypersensitivity in the Lungs of Mice Infected with an Influenza A Virus
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感染甲型流感病毒的小鼠肺部介导迟发型超敏反应的细胞
DOI：
10.1111/j.1365-3083.1980.tb00083.x
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发表时间：
1980
影响因子：
3.7
通讯作者：
G. Ada
中科院分区：
文献类型：
--
作者：
K. N. Leung;G. Ada
在6天前鼻内接种甲型流感病毒致死剂量（通常为5 × 104EID50）的小鼠肺部，可以恢复在抗原转移到幼稚小鼠时表现出延迟型超敏反应（DTH）的效应细胞。恢复的活性与鼻内注入病毒的剂量和观察到的肺实变程度成正比。活性细胞也可以从引流淋巴结和外周血中恢复。效应细胞被确定为Ly I型T淋巴细胞，并且需要在供体和受体之间共享I区才能激发活性。它们在A组流感病毒中发生交叉反应。据报道，在两个实验中，表达DTH活性但细胞毒性T细胞活性非常低的免疫细胞制剂被转移到1或2天前接种致死剂量病毒的小鼠身上。老鼠并没有免于死亡，在两个实验中，接受治疗的老鼠比对照组死得更快。这些结果与先前的结果形成对比，先前的结果显示，具有高细胞毒性T细胞活性的细胞制剂可保护受感染小鼠免于死亡。
Effector cells that demonstrate delayed‐type hypersensitivity (DTH) on transfer with antigen to naive mice can he recovered from the lungs of mice inoculated intranasally 6 days earlier with a lethal dose (usually 5 × 104EID50) of influenza A virus. The activity recovered was proportional to the dose of virus instilled intranasally and the extent of lung consolidation observed. Active cells could also be recovered from the draining lymph nodes and from the peripheral blood. The effector cells were identified as T lymphocytes of Ly I phenotype and required I‐region sharing between donor and recipient for activity to be elicited. They were cross‐reactive within the A group of influenza viruses. Two experiments are reported in which immune cell preparations that expressed DTH activity but had very little cytotoxic T cell activity were transferred to mice inoculated 1 or 2 days earlier with a lethal dose of virus. The mice were not protected from death, and in both experiments, the recipient mice died more rapidly than the controls. These results contrast with earlier results in which cell preparations with high cytotoxic T‐cell activity were shown to protect recipient infected mice from death.