Simultaneous measurement of aldosterone and cortisol by high-performance liquid chromatography-tandem mass spectrometry: Application to dehydration-rehydration studies
Simultaneous measurement of aldosterone and cortisol by high-performance liquid chromatography-tandem mass spectrometry: Application to dehydration-rehydration studies
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DOI：
10.1016/j.jchromb.2010.03.033
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发表时间：
2010-05-01
影响因子：
3
通讯作者：
Stowasser, Michael
中科院分区：
文献类型：
--
作者：
Taylor, Paul J.;van Rosendal, Simon P.;Stowasser, Michael
醛固酮和皮质醇分别是脱水和压力的有用生物标志物。本研究的目的是开发一种高效液相色谱-串联质谱方法，用于同时测量人血浆中的醛固酮和皮质醇，该方法可应用于运动员运动和补液的研究。使用与三重四极杆串联质谱仪联用的在线样品制备/HPLC 系统制备和分析样品。样品（200 μL）在 Hysphere C18 HD 柱（7 μm，Spark Holland）上进行预处理和提取。在等度条件下以 0.3 mL/min 的流速在 Sunfire C18 分析柱（50 mm x 3.0 mm，3 μm，Waters）上进行色谱分析。流动相由 35% 乙腈/水组成。质谱检测是通过使用负电喷雾电离条件的选择反应监测来进行的。该测定的醛固酮和皮质醇的分析范围分别为 25-500 pg/mL 和 25-500 ng/mL (r(2) > 0.992。n = 22)。质量控制样品的日间准确度和不精密度为 99.4-106%
Aldosterone and cortisol are useful biomarkers of dehydration and stress, respectively. The aim of this study was to develop an HPLC-tandem mass spectrometric method for the simultaneous measurement of aldosterone and cortisol in human plasma that could be applied to the study of athletes undergoing exercise and rehydration. Samples were prepared and analysed using an on-line sample preparation/HPLC system coupled to a triple quadrupole tandem-mass spectrometer. Samples (200 mu L) were pre-treated and extracted on Hysphere C18 HD cartridges (7 mu m, Spark Holland). Chromatography was performed on a Sunfire C18 analytical column (50 mm x 3.0 mm, 3 mu m, Waters) under isocratic conditions at a flow rate of 0.3 mL/min. The mobile phase consisted of 35% acetonitrile/water. Mass spectrometric detection was by selected reaction monitoring using negative electrospray ionization conditions. The assay had an analytical range of 25-500 pg/mL and 25-500 ng/m L for aldosterone and cortisol, respectively (r(2) > 0.992. n = 22). Inter-day accuracy and imprecision for quality control samples was 99.4-106% and