Sterilization of Streptococcus pyogenes by afterglow dielectric barrier discharge using O2 and CO2 working gases
Sterilization of Streptococcus pyogenes by afterglow dielectric barrier discharge using O2 and CO2 working gases
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DOI：
10.1016/j.bej.2010.06.017
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发表时间：
2010-09-15
影响因子：
3.9
通讯作者：
Omran, Azadeh Valinataj
中科院分区：
文献类型：
--
作者：
Colagar, Abasalt Hosseinzadeh;Sohbatzadeh, Farshad;Omran, Azadeh Valinataj
化脓性链球菌是多种人类疾病的病原体。为了通过介电屏障放电 (DBD) 的余辉灭活化脓性链球菌，我们在液体（浓度为 OD600 nm = 0.25 麦克法兰标准）和固体（每个表面板有 7.75 x 10(5) 个扩散细胞）Luria-Bertini (LB) 肉汤培养基中制备了培养细菌的悬浮液。在 5、10 和 15 分钟时，通过两种方法评估 DBD 暴露对化脓性链球菌的影响：比浊法分析（对于液体溶液）和确定菌落形成单位数（对于固体培养基）。细菌的存活很大程度上取决于所使用的工作气体。对于培养的 LB 液体溶液，在 O-2 和 CO2 工作气体暴露 15 分钟时，其分别达到 (21 +/- 5)% 和 (70 +/- 8)%。对于 O-2 和 CO2 气体，LB 板表面上的细菌细胞生长分别为 1 个菌落和 270 个菌落。结果表明，O-2 气体的 DBD 可有效消毒液体和表面培养基中的化脓性链球菌。该过程可归因于臭氧和激发单线态O-2(a(1) Delta(g))作为活性化学试剂。 (C) 2010 Elsevier B.V. 保留所有权利。
Streptococcus pyogenes is a causative agent in wide range of human diseases. To inactivate S. pyogenes by the afterglow of a dielectric barrier discharge (DBD), we prepared suspensions of the cultured bacterium in liquid (with concentrations of OD600 nm = 0.25 McFarland standard) and solid (with 7.75 x 10(5) spread cells per surface plate) Luria-Bertini (LB) broth media. The influence of DBD exposure on S. pyogenes was evaluated at 5, 10, and 15 min by two methods: turbidimetry analysis (for liquid solution) and determination of the number of colony-forming units (for solid media). Bacterial survival was strongly dependent upon on the working gas used. For a cultured LB liquid solution, it reached (21 +/- 5)% and (70 +/- 8)% at an exposure time of 15 min with O-2 and CO2 working gases, respectively. Bacterial cell growth on the surface of the LB plate was 1 colony and 270 colonies for O-2 and CO2 gases, respectively. The results suggest that the DBD of O-2 gas could be efficient in the disinfection of S. pyogenes in liquid and surface cultured media. The processes could be attributed to ozone and the excited singlet state O-2(a(1) Delta(g)) as the active chemical agents. (C) 2010 Elsevier B.V. All rights reserved.