A comparative study of immunohistochemical methods for detecting abnormal prion protein with monoclonal and polyclonal antibodies
A comparative study of immunohistochemical methods for detecting abnormal prion protein with monoclonal and polyclonal antibodies
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DOI：
10.1053/jcpa.1999.0343
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发表时间：
2000-01-01
影响因子：
0.8
通讯作者：
Groschup, MH
中科院分区：
文献类型：
--
作者：
Hardt, M;Baron, T;Groschup, MH
传染性海绵状脑病与中枢神经系统异常朊蛋白（PrPSc）的积累有关，可通过免疫组织化学检测。使用反刍动物PrP第一α -螺旋表位的单克隆抗体（L42），我们比较了先前报道的免疫组织化学抗原揭露和“可视化”系统。此外，还对反刍动物PrP其他表位的多种多克隆和单克隆抗体进行了评估。通过水合高压灭菌和蛋白酶K处理，以及L42和亲和素-生物素复合物系统进行抗原检测，可以在携带三种不同PrP等位基因的羊以及牛海绵状脑病病例中证明神经元内和神经元外PrPSc：。(C) 2000 Harcourt出版社有限公司
Transmissible spongiform encephalopathies are associated with the accumulation of abnormal prion protein (PrPSc) in the central nervous system which can be detected immunohistochemically. Using a monoclonal antibody (L42) to an epitope on the first alpha-helix of ruminant PrP, we compared previously reported immunohistochemical antigen unmasking and "visualization" systems. Tn addition, a variety of polyclonal and monoclonal antibodies to other epitopes:on ruminant PrP were assessed. Antigen unmasking by hydrated autoclaving and proteinase K treatments, and antigen detection with L42 and an avidin-biotin complex system, enabled intra- and extra-neuronal PrPSc:to be demonstrated in scrapie-affected sheep carrying three different PrP alleles, as well as in cases of bovine spongiform encephalopathy. (C) 2000 Harcourt Publishers Ltd.