Tracking eastern equine encephalitis virus perpetuation in the northeastern United States by phylogenetic analysis
Tracking eastern equine encephalitis virus perpetuation in the northeastern United States by phylogenetic analysis
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DOI：
10.4269/ajtmh.2008.79.291
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发表时间：
2008-08-01
影响因子：
3.3
通讯作者：
Mores, Christopher N.
中科院分区：
文献类型：
--
作者：
Armstrong, Philip M.;Andreadis, Theodore G.;Mores, Christopher N.
东部马脑炎病毒（EEEV）的流行和动物流行病在温带地区零星发生，传播从夏末到初秋是季节性的。这些暴发可能源于在当地越冬的病毒，也可能源于从其他地点重新引入的病毒。为了评估这些可能性，我们比较了从康涅狄格州全州虫媒病毒监测期间收集的蚊子中分离出的EEEV分离株的系统发育关系，以及从新罕布什尔州南部和纽约州北部同时爆发的分离株。在康涅狄格，病毒分离物按分离年份或超过2年的采样时间分成暂时离散的分支。2000年、2001年、2003年、2004年和2006年出现了两个或更多的分支，可能是不同的传入事件的结果，而来自纽约州北部和新罕布什尔州的病毒分离成一个分支，持续了2年或更长时间。新罕布什尔州的病毒与康涅狄格州分离出的病毒有着最近的共同祖先，这表明病毒在这些地区传播。这些结果为eev在北部疫源地的独立越冬事件提供了额外的证据。
Epidemics and epizootics of eastern equine encephalitis virus (EEEV) occur sporadically in temperate regions where transmission is seasonal from late summer to early fall. These outbreaks may derive from virus that overwinters locally or perhaps results from reintroduction of virus from other sites. To evaluate these possibilities, we compared the phylogenetic relationships of EEEV isolates obtained from mosquitoes collected during statewide arbovirus surveillance in Connecticut, in addition to isolates from concurrent outbreaks in southern New Hampshire and upstate New York. In Connecticut, viral isolates grouped into temporally discrete clades by year of isolation or over 2 years of sampling. Two or more clades arose in 2000, 2001, 2003, 2004, and 2006, possibly the result of separate introduction events into the state, whereas viruses from upstate New York and New Hampshire segregated into single clades that persisted for 2 or more years. New Hampshire viruses shared recent common ancestry to those isolated in Connecticut suggesting viral dispersal among these regions. These results provide additional evidence for independent episodes of EEEV overwintering in northern foci.