Characterization of acellular dermal matrices (ADMs) prepared by two different methods
Characterization of acellular dermal matrices (ADMs) prepared by two different methods
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DOI：
10.1016/s0305-4179(97)00110-1
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发表时间：
1998-03-01
期刊：
影响因子：
2.7
通讯作者：
Hanumadass, M
中科院分区：
文献类型：
--
作者：
Walter, RJ;Matsuda, T;Hanumadass, M
脱细胞真皮基质（ADM）作为真皮替代物治疗全层皮肤损伤的有效性取决于其低抗原性、快速血管化能力和作为真皮模板的稳定性。这些特性在很大程度上取决于adm的最终组成。我们用disase和Triton X-100洗涤剂或NaCl和SDS洗涤剂处理人体皮肤，对所得adm进行冷冻切片，然后用免疫组织化学方法对其进行表征。两种方法制备的adm中细胞相关抗原（HLA-ABC、HLA-DR、vimentin、desmin、talin）、细胞外基质成分（硫酸软骨素、纤维连接蛋白、层粘连蛋白、玻璃体连接蛋白、透明质酸）、弹性蛋白和VII型胶原的染色均显著减少或缺失。然而，在两种adm中仍观察到大量的弹性蛋白、硫酸角蛋白、层粘连蛋白和III型和IV型胶原。两种制备ADM的方法都能广泛提取皮肤的细胞和细胞外成分，但保留了皮肤的基本结构。总体而言，NaCl-SDS法制备的ADM各抗原保留量均大于Dispase-Triton法制备的ADM。这在层粘连蛋白和VII型胶原蛋白中最为明显，但在NaCl-SDS adm中也发现了大量的IV型胶原蛋白、纤维连接蛋白、去丝蛋白、弹性蛋白和HLA-DR。(C) 1998年Elsevier Science Ltd为ISBI出版。版权所有。
The efficacy of acellular dermal matrix (ADM) in the treatment of full-thickness skin injuries as a dermal substitute depends on its low antigenicity, capacity for rapid vascularization, and stability as a dermal template. These properties will be determined largely by the final composition of the ADM. We have treated human skin with either Dispase followed by Triton X-100 detergent or NaCl followed by SDS detergent, cryosectioned the resulting ADMs, and then characterized them immunohistochemically. Staining for cell-associated antigens (HLA-ABC, HLA-DR, vimentin, desmin, talin), extracellular matrix components (chondroitin sulfate, fibronectin, laminin, vitronectin, hyaluronic acid), elastin, and collagen type VII was dramatically reduced or absent from ADMs prepared by both methods. However, significant amounts of elastin, keratan sulfate, laminin, and collagen types III and IV were still observed in both ADMs. Both methods of ADM preparation resulted in extensive extraction of both cellular and extracellular components of the skin but retention of the basic dermal architecture. In general, ADM prepared by the NaCl-SDS method retained larger amounts of each antigen than did that prepared by the Dispase-Triton method. This was most evident for laminin and type VII collagen but larger amounts of type IV collagen, fibronectin, desmin, elastin, and HLA-DR were also evident in the NaCl-SDS ADM. (C) 1998 Published by Elsevier Science Ltd for ISBI. All rights reserved.