Influence of Gibberellic Acid on Growth and Endogenous Auxin Level in Epicotyl and Hypocotyl Tissues of Normal and Dwarf Bean Plants
Influence of Gibberellic Acid on Growth and Endogenous Auxin Level in Epicotyl and Hypocotyl Tissues of Normal and Dwarf Bean Plants
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DOI：
10.1111/j.1399-3054.1976.tb04862.x
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发表时间：
1976-10
影响因子：
6.4
通讯作者：
K. Jindal;T. Hemberg
中科院分区：
文献类型：
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作者：
K. Jindal;T. Hemberg
在矮菜豆植物（cv. Alabaster）的顶端施用赤霉酸刺激了上胚轴和下胚轴的伸长生长，但对正常品种（“蓝湖”）的伸长生长没有显着影响。赤霉素处理矮化植物的特征是内源生长素水平增加约两倍。 GA 处理 48 小时后观察到 IAA 水平最大增加。正常豆类植物中 IAA 含量增加较少。 — 对矮化或正常品种的切除的上胚轴和下胚轴切片进行赤霉素处理，显示对伸长生长没有影响。然而，在矮化品种的切片中观察到生长素水平显着增加。在含有赤霉素的基础培养基中仅孵育 1 小时即可产生最大效果。 — 采用吲哚-α-吡喃酮分光荧光法测定IAA。
Application of gibberellic acid to the apex of dwarf bean plants (cv. Alabaster) stimulated the elongation growth of epicotyl and hypocotyl but showed no significant effect on elongation growth in a normal cultivar (‘Blue Lake’). Gibberellin-treatment of dwarf plants was characterized by about twofold increase in the level of endogenous auxin. Maximum increase in IAA level was observed after 48 h of GA treatment. There was less increase in IAA content in normal bean plants. — Gibberellin treatment to excised epicotyl and hypocotyl sections of either dwarf or normal cultivar showed no effect on elongation growth. However, a considerable increase in the auxin level was observed in the sections of the dwarf cultivar. The maximum effect occurred with only 1 h incubation in basal medium containing gibberellin. — The indolo-α-pyrone spectro-fluoremetric method for IAA determination was used.