The full-scale anaerobic digestion microbiome is represented by specific marker populations
The full-scale anaerobic digestion microbiome is represented by specific marker populations
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DOI：
10.1016/j.watres.2016.08.008
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发表时间：
2016-11-01
期刊：
影响因子：
12.8
通讯作者：
Boon, Nico
中科院分区：
文献类型：
--
作者：
De Vrieze, Jo;Raport, Linde;Boon, Nico
厌氧消化是一种成熟的微生物技术，用于处理有机废物流和随后的沼气回收。一个强大且多功能的微生物群落对于确保过程的整体稳定性至关重要。对四个全尺寸厌氧消化厂进行了为期一年的跟踪，以将运行特征与微生物群落的组成和结构联系起来。分析了消化池之间的相似性、群落动态以及每个消化池内细菌和古细菌之间的共现情况。游离氨浓度(>200 mg N·L-1)和电导率(>30 mS cm(-1))阻碍了甲烷菌的乙酰分解产甲烷作用。因此，产甲烷作用被推向由甲烷细菌目和甲烷微生物目进行的氢营养途径。厚壁菌门在每个消化池中的整体细菌群落中占主导地位（> 50%），然而，Bray-Curtis 指数的主坐标分析表明，四个消化池中的每一个都有一个独特的微生物群落。该群落的独特性与属于互养单胞菌属的两种系统型（Phy32 和 Phy34）和一种未分类细菌（Phy2）有关，它们都可以被视为该群落中的标记种群。在不同消化器之间观察到产甲烷菌与几种细菌共存的明显差异。我们的结果表明，全尺寸厌氧消化装置在时间函数中表现出恒定的动态和共现模式，但在组成方面是独特的，与标记群体的存在有关。 (C) 2016 Elsevier Ltd. 保留所有权利。
Anaerobic digestion is a well-established microbial-based technology for the treatment of organic waste streams and subsequent biogas recovery. A robust and versatile microbial community to ensure overall stability of the process is essential. Four full-scale anaerobic digestion plants were followed for one year to link operational characteristics with microbial community composition and structure. Similarities between digesters, community dynamics and co-occurrence between bacteria and archaea within each digester were analysed. Free ammonia concentration (>200 mg N L-1) and conductivity (>30 mS cm(-1)) hindered acetoclastic methanogenesis by Methanosaetaceae. Thus, methanogenesis was pushed to the hydrogenotrophic pathway carried out by Methanobacteriales and Methanomicrobiales. Firmicutes dominated the overall bacterial community in each of the digesters (>50%), however, principal coordinate analysis of Bray-Curtis indices showed that each of the four digesters hosted a unique microbial community. The uniqueness of this community was related to two phylotypes belonging to the Syntrophomonas genus (Phy32 and Phy34) and to one unclassified bacterium (Phy2), which could both be considered marker populations in the community. A clear differentiation in co-occurrence of methanogens with several bacteria was observed between the different digesters. Our results demonstrated that full-scale anaerobic digestion plants show constant dynamics and co-occurrence patterns in function of time, but are unique in terms of composition, related to the presence of marker populations. (C) 2016 Elsevier Ltd. All rights reserved.