The minimal functional sequence of protamine
The minimal functional sequence of protamine
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DOI：
10.1016/j.bbrc.2005.08.151
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发表时间：
2005-10-21
影响因子：
3.1
通讯作者：
Vaynshteyn, Y
中科院分区：
文献类型：
--
作者：
Liang, JF;Yang, VC;Vaynshteyn, Y
尽管鱼精蛋白的应用几乎是普遍的，但它是四种不同序列的主要肽的混合物，与临床显著的副作用有关。通过精心设计的酶切方法，得到了各种低分子量的脯胺肽。其中，通过体外和体内实验，鉴定出两种低分子量的脯胺肽，它们与天然脯胺具有相同甚至更强的肝素中和能力。此外，体内实验表明，与天然脯胺相比，这两种低分子量的鱼精蛋白肽毒性更小，临床应用更安全。(c) 2005爱思唯尔公司版权所有。
Despite its nearly universal applications, protamine, a mixture of four major peptides with different sequences, is associated with clinically significant side effects. Through a well-designed enzyme digestion method, various low molecular weight prolamine peptides were obtained. Among them, two low molecular weight prolamine peptides with the same or even more potent heparin neutralization abilities as native prolamine were identified through both in vitro and in vivo tests. In addition, in vivo experiments showed that compared to native prolamine, these two low molecular weight protamine peptides were less toxic and would be safer for clinical use. (c) 2005 Elsevier Inc. All rights reserved.