A type II polyketide synthase is responsible for anthraquinone biosynthesis in Photorhabdus luminescens
A type II polyketide synthase is responsible for anthraquinone biosynthesis in Photorhabdus luminescens
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DOI：
10.1002/cbic.200700300
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发表时间：
2007-09-24
期刊：
影响因子：
3.2
通讯作者：
Bode, Helge B.
中科院分区：
文献类型：
--
作者：
Brachmann, Alexander O.;Joyce, Susan A.;Bode, Helge B.
II型聚酮化合物脱氢酶参与许多具有强效抗生素或抗癌活性的临床相关次级代谢物的生物合成。直到最近，唯一已知的II型PKS的生产者是革兰氏阳性放线菌的成员，通常是众所周知的次级代谢产物的生产者。在这里，我们提出了来自革兰氏阴性菌的II型PKS的第二个例子。我们已经确定了生物合成基因簇负责生产蒽醌（AQs）的昆虫病原细菌发光杆菌。这是在革兰氏阴性菌中生产AQ的第一个实例，并且它们的heptaketide来源通过喂养实验证实。缺失的环化酶/芳香化酶参与AQ生物合成导致积累的mutactin和dehydromutactin，这已被描述为分流产品的典型octaketide化合物从链霉菌，AQ形成从octaketide中间体的途径进行了讨论。
Type II polyketide synthases are involved in the biosynthesis of numerous clinically relevant secondary metabolites with potent antibiotic or anticancer activity. Until recently the only known producers of type II PKSs were members of the Gram-positive actimomycetes, well-known producers of secondary metabolites in general. Here we present the second example of a type II PKS from Gram-negative bacteria. We have identified the biosynthesis gene cluster responsible for the production of anthraquinones (AQs) from the entomopathogenic bacterium Photorhabdus luminescens. This is the first example of AQ production in Gram-negative bacteria, and their heptaketide origin was confirmed by feeding experiments. Deletion of a cyclase/aromatase involved in AQ biosynthesis resulted in accumulation of mutactin and dehydromutactin, which have been described as shunt products of typical octaketide compounds from streptomycetes, and a pathway for AQ formation from octaketide intermediates is discussed.