Selective reduction of c-myc mRNA in Daudi cells by human beta interferon.
Selective reduction of c-myc mRNA in Daudi cells by human beta interferon.
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人 β 干扰素选择性减少 Daudi 细胞中的 c-myc mRNA。
DOI：
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发表时间：
1984
影响因子：
11.1
通讯作者：
E. Knight
中科院分区：
文献类型：
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作者：
G. Jonak;E. Knight
在正常生长条件下，人淋巴母细胞样细胞系Daudi表达高水平的c-myc mRNA。这些细胞对干扰素的生长抑制也很敏感。我们通过RNA点印迹和印迹杂交分析方法比较了未经处理和人β干扰素（ifn - β）处理的Daudi细胞中c-myc mRNA的水平。使用与人类c-myc mRNA互补的合成寡核苷酸作为探针，我们检测到ifn - β处理的细胞中c-myc可杂交多(a)+ RNA减少了75%以上。这种c-myc mRNA的减少似乎是选择性的，因为肌动蛋白mRNA的水平不受ifn - β治疗的显著影响。此外，从ifn - β处理的细胞中提取的mRNA的体外翻译和ifn - β处理的细胞中细胞蛋白的体内合成都没有定量影响。我们推测，在ifn - β处理的Daudi细胞中，c-myc mRNA数量的选择性减少可能与ifn诱导的抑制Daudi肿瘤细胞生长有关。
Under normal growth conditions, the human lymphoblastoid cell line Daudi expresses high levels of c-myc mRNA. These cells are also sensitive to growth inhibition by interferons. We have compared the levels of mRNA for the c-myc in untreated and human beta interferon (IFN-beta)-treated Daudi cells by RNA dot-blot and blot-hybridization analysis methods. Using a synthetic oligonucleotide complementary to the human c-myc mRNA as the probe, we detected a more than 75% reduction in the c-myc hybridizable poly(A)+ RNA in the IFN-beta-treated cells. This reduction in the c-myc mRNA appears to be selective because the level of actin mRNA is not significantly affected by the IFN-beta treatment. In addition, neither in vitro translation of mRNA extracted from IFN-beta-treated cells nor in vivo synthesis of cellular proteins in IFN-beta-treated cells are quantitatively affected. We surmise that the selective reduction in the amount of c-myc mRNA in IFN-beta-treated Daudi cells may be related to the IFN-induced inhibition of the Daudi tumor cell growth.