Microparticles in sputum of COPD patients: a potential biomarker of the disease?
Microparticles in sputum of COPD patients: a potential biomarker of the disease?
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DOI：
10.2147/copd.s99547
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发表时间：
2016
影响因子：
2.8
通讯作者：
Porro C
中科院分区：
文献类型：
--
作者：
Lacedonia D;Carpagnano GE;Trotta T;Palladino GP;Panaro MA;Zoppo LD;Foschino Barbaro MP;Porro C
微粒子(MPS)是细胞在化学、物理和细胞凋亡刺激下释放的0.1-1微米的膜小泡。MPS代表的不仅仅是细胞的微型版本。它们的组成和功能不仅取决于细胞来源，还取决于刺激。慢性阻塞性肺疾病(COPD)是一种肺部疾病，其特征是几乎不可逆转的肺破坏，导致呼吸道受限。我们调查了COPD患者痰中MPS的存在和来源，以评估MP数量和来源的变化是否可以反映疾病的病理生理状况，并可能作为诊断和预后的潜在生物标志物。取18例男性受试者的诱导痰标本，用Sputasol液化。免疫标记白细胞(CD11a)、粒细胞(CD66b)、单核巨噬细胞(CD11b)、血小板和巨核细胞(CD41)、内皮细胞(CD31)和红细胞(CD235ab)，并用细胞荧光法进行分析。CD3 1-MPS与1s用力呼气量呈负相关(R=−5 3，P<0.0 5)，CD66b-MP水平与COPD患者的体重指数、气流障碍、呼吸困难、运动耐量(BODE)呈正相关，与6min步行试验呈负相关(P<0.0 5)，与−呈负相关(P<0.0 5)。CD235ab-MPS与体重指数呈负相关(R=−0.86，P<0.05)，与呼吸困难指数呈正相关(R=0.91，P<0.05)。本研究的主要发现是在受COPD影响的患者的痰中检测到MPS。部分患者的表型与COPD的主要参数有关。提示MPS可能参与了COPD的发病机制。
Microparticles (MPs) are small membrane vesicles of 0.1–1 µm which are released by cells following chemical, physical, and apoptotic stimuli. MPs represent more than a miniature version of the cell. Their composition and function depend not only on cellular origin, but also on stimuli. Chronic obstructive pulmonary disease (COPD) is a lung disease characterized by nearly irreversible lung destruction which results in airway limitation. We investigated the presence and source of MPs in sputum of COPD patients to evaluate if changes in MP number and origin may reflect the pathophysiological conditions of disease and may serve as potential biomarkers for diagnostic and prognostic use. Induced sputum samples were collected from 18 male subjects and liquefied with Sputasol. MPs obtained were immunolabeled for leukocyte (CD11a), granulocyte (CD66b), monocyte-macrophage (CD11b), platelets and megakaryocytic cells (CD41), endothelial cells (CD31), and red blood cells (CD235ab) and analyzed by cytofluorimetry. There was a negative correlation between CD31-MPs and forced expiratory volume in 1 second (R=−53, P<0.05) and CD66b-MP level was correlated with worse performance index of COPD such as the Body mass index airflow Obstruction, Dyspnea, and Exercise capacity (BODE); they were negatively correlated with 6-minute walking test: 0.65 and −0.64, respectively (P<0.05). CD235ab-MPs showed a negative correlation with body mass index (R=−0.86, P<0.05), while there was a positive correlation with dyspnea index (R=0.91, P<0.05). The main finding of this study was that MPs were detected in the sputum of patients affected by COPD. The phenotype of some of them was related to the main COPD parameters. These results suggest that MPs could be implicated in the pathogenesis of COPD.