Cascades of transcriptional induction during human lymphocyte activation
Cascades of transcriptional induction during human lymphocyte activation
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DOI：
10.1078/0171-9335-00162
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发表时间：
2001-05-01
影响因子：
6.6
通讯作者：
Haber, DA
中科院分区：
文献类型：
--
作者：
Ellisen, LW;Palmer, RE;Haber, DA
已知淋巴细胞活化与涉及细胞因子信号传导和细胞增殖的基因的诱导相关。高密度微阵列提供了监测整体细胞表达谱、转录物类别之间的时间关系以及与人类疾病或免疫抑制相关的改变的手段。我们试图确定微阵列分析是否能准确反映人类T细胞活化后基因表达的正常模式，以及是否可以分析鉴定的复杂表达模式以产生淋巴细胞活化的功能谱。我们研究了伴刀豆球蛋白A激活的人淋巴细胞中6800个细胞转录物的时序表达谱。使用聚类分析对表达模式进行分组，并使用北方印迹进行验证。准确鉴定了已知在T细胞活化后诱导的基因，并且基因表达的定性模式在北方和微阵列分析之间具有良好的相关性。基因表达水平的定量差异在这两种技术之间的相关性较低。表达谱分析揭示了功能相似基因组的顺序诱导，其时间协同调节强调了T细胞活化过程中已知的细胞事件。淋巴细胞活化的这种功能“指纹”可能被证明对比较实验条件下和疾病状态下的淋巴细胞反应有用。
Lymphocyte activation is known to be associated with the induction of genes implicated in cytokine signaling and cellular proliferation. High-density microarrays offer the means to monitor global cellular expression profiles, temporal relationships between classes of transcripts, and alterations associated with human disease or immunosuppression, We sought to determine whether microarray analysis would accurately reflect the normal pattern of gene expression following human T cell activation, and whether the complex expression patterns identified could be analyzed to produce a functional profile of lymphocyte activation. We examined a time course of sequential expression profiles for 6800 cellular transcripts in human lymphocytes activated with concanavalin A. Expression patterns were grouped using clustering analysis and validated using Northern blotting. Genes known to be induced following T cell activation were accurately identified, and the qualitative patterns of gene expression were well correlated between Northern and microarray analyses. Quantitative differences in gene expression levels were less well correlated between these two techniques, Expression profile analysis revealed the sequential induction of groups of functionally similar genes, whose temporal coregulation underscores known cellular events during T cell activation, This functional "fingerprint" of lymphocyte activation may prove useful for comparisons of lymphocyte responses under experimental conditions and in disease states.