A new method for measurement of blood pressure, heart rate, and activity in the mouse by radiotelemetry
A new method for measurement of blood pressure, heart rate, and activity in the mouse by radiotelemetry
复制标题
DOI：
10.1152/jappl.2000.88.5.1537
复制
发表时间：
2000-05-01
影响因子：
3.3
通讯作者：
Kramer, K
中科院分区：
文献类型：
--
作者：
Mills, PA;Huetteman, DA;Kramer, K
开发并验证了一种简单可靠的方法，用于准确、长期测量有意识、自由活动的实验室小鼠的脉搏血压（BP）。新开发的设备包括一个小的（1.9英里，3.4克），完全植入式无线电遥测发射器。初始频率响应测试显示有足够的动态响应；在五个发射机中发现的平均- 3db点为145 +/- 14 (SD) Hz。记录16只长期（30-150天）植入小鼠的血压、心率和运动活动。在整个研究过程中，定期检查的平均动脉压和脉压分别在90-140毫米汞柱和30-50毫米汞柱之间。变送器BP测量结果在Millar 1.4 fr下进行了验证。transducer-tipped导管。舒张压传递器的平均误差为+1.1 +/- 6.9 mmHg （n = 7）。收缩压的误差平均大2.7±3.9 mmHg。在至少150天的时间里，这种新设备可以准确地监测有意识、不受束缚、自由活动的老鼠的血压、心率和运动活动。
A simple and reliable means for accurate, chronic measurement bf pulsatile blood pressure (BP) from conscious, freely moving laboratory mice was developed and validated. The newly developed device consists of a small (1.9 mi, 3.4 g), fully implantable radiotelemetry transmitter. Initial frequency response tests showed an adequate dynamic response; the average -3-dB point found in five transmitters was 145 +/- 14 (SD) Hz. BP, heart rate, and locomotor activity were recorded from 16 chronically (30-150 days) implanted mice. Mean arterial and pulse pressure, checked at regular intervals, ranged from 90-140 mmHg and from 30-50 mmHg, respectively, throughout the study. Transmitter BP measurements were validated against a Millar 1.4-Fr. transducer-tipped catheter. The mean error of the transmitters for diastolic pressures was +1.1 +/- 6.9 mmHg (n = 7). The error for systolic pressures was, on average, 2.7 +/- 3.9 mmHg larger. This new device accurately monitors BP, heart rate, and locomotor activity in conscious, untethered, freely moving mice living in their home cages for periods of at least 150 days.