Enantioselective open-tubular capillary electrochromatography using a β-cyclodextrin–gold nanoparticles–polydopamine coating as a stationary phase
Enantioselective open-tubular capillary electrochromatography using a β-cyclodextrin–gold nanoparticles–polydopamine coating as a stationary phase
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DOI：
10.1039/c8nj03234c
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发表时间：
2018-10
影响因子：
3.3
通讯作者：
Li Zhou;Bo Zhang;Shuang Li;Jia Yu;Xingjie Guo
中科院分区：
文献类型：
--
作者：
Li Zhou;Bo Zhang;Shuang Li;Jia Yu;Xingjie Guo
以巯基β-环糊精（SH-β-CD）偶联聚多巴胺-金纳米粒子（PDA-AuNPs）为固定相，建立了一种新型的对映选择性开管毛细管电色谱（OT-CEC）技术。毛细管内表面官能化多巴胺通过自聚合在碱性条件下，随后引入金纳米粒子的基础上的静电相互作用和亲和力的金纳米粒子和胺基团的聚多巴胺。然后通过S-Au共价键将巯基β-环糊精引入到金纳米颗粒层上。优化了多巴胺浓度、金纳米粒子包覆层数和巯基β-环糊精浓度等制备条件，获得了较好的对映选择性。利用扫描电镜（SEM）、能谱仪（EDS）和透射电镜（TEM）对所制备的开管柱进行了表征。通过对色氨酸、苯丙氨酸、组氨酸、非索非那定、异丙嗪、托吡卡胺和特布他林等7种手性药物的分离，验证了开管柱的分离效果，获得了满意的分离结果。此外，通过日内、日间和柱间考察，对色谱柱的稳定性和重复性进行了研究。
A novel enantioselective open-tubular capillary electrochromatography (OT-CEC) technique was developed employing thiol β-cyclodextrin (SH-β-CD) conjugated polydopamine–gold nanoparticles (PDA–AuNPs) as a stationary phase. The capillary inner surface was functionalized with dopamine by self-polymerization under alkaline conditions and the subsequent introduction of gold nanoparticles based on the electrostatic interaction and affinity between gold nanoparticles and amine groups on polydopamine. Then, thiol β-cyclodextrin was introduced on the gold nanoparticle layer through S–Au covalent bonding. The preparation conditions including dopamine concentration, the number of coating layers of gold nanoparticles and thiol β-cyclodextrin concentration were optimized to obtain satisfactory enantioselectivity. Scanning electron microscopy (SEM), energy dispersive X-ray analysis spectroscopy (EDS) and EOF experiments were carried out to characterize the prepared open tubular (OT) column. Then, the separation effectiveness of the open tubular column was verified by the separation of seven chiral drugs including tryptophan, phenylalanine, histidine, fexofenadine, promethazine, tropicamide and terbutaline, and satisfactory separation results were obtained for the analytes studied. Moreover, the stability and repeatability of the column were studied through intra-day, inter-day and column-to-column investigations.