Local and systemic wound-induction of RNase and nuclease activities in Arabidopsis:: RNS1 as a marker for a JA-independent systemic signaling pathway
Local and systemic wound-induction of RNase and nuclease activities in Arabidopsis:: RNS1 as a marker for a JA-independent systemic signaling pathway
复制标题
DOI：
10.1046/j.1365-313x.2002.01223.x
复制
发表时间：
2002-02-01
期刊：
影响因子：
7.2
通讯作者：
Green, PJ
中科院分区：
文献类型：
--
作者：
LeBrasseur, ND;MacIntosh, GC;Green, PJ
诱导防御相关基因是植物对机械伤害做出反应的一种方式。我们研究了RNase是否参与了拟南芥的伤害反应。与其他植物系统一样，在拟南芥受损的叶片、茎和幼苗中，至少有三种能够降解RNA和DNA的双功能核酸酶以及普遍存在的RNase T-2家族成员RNS1被诱导具有不同的活性。RNS1的强烈诱导特别有趣，因为它发生在创伤后的局部和全身。这种核糖核酸酶的系统诱导表明，该家族的成员除了参与先前假设的营养循环和再动员功能外，还可能参与防御机制。此外，系统的诱导似乎独立于茉莉酸控制，RNS1的局部诱导和核酸酶活性独立于JA和寡糖激发子。因此，在拟南芥中似乎存在一种新的系统途径，可能涉及第三种信号。
Induction of defense-related genes is one way in which plants respond to mechanical injury. We investigated whether RNases are involved in the wound response in Arabidopsis thaliana. As in other plant systems, several activities are induced with various timings in damaged leaves, stems and seedlings in Arabidopsis, including at least three bifunctional nucleases, capable of degrading both RNA and DNA, as well as RNS1, a member of the ubiquitous RNase T-2 family of RNases. The strong induction of RNS1 is particularly interesting because it occurs both locally and systemically following wounding. The systemic induction of this RNase indicates that members of this family may be involved in defense mechanisms in addition to their previously hypothesized functions in nutrient recycling and remobilization. Additionally, the systemic induction appears to be controlled independently of jasmonic acid, and the local induction of RNS1 and the nuclease activities are independent of both JA and oligosaccharide elicitors. Consequently, a novel systemic pathway, likely involving a third signal, appears to exist in Arabidopsis.