Single-Molecule Spectroscopy on RC-LH1 Complexes of Rhodopseudomonas acidophila Strain 10050
Single-Molecule Spectroscopy on RC-LH1 Complexes of Rhodopseudomonas acidophila Strain 10050
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DOI：
10.1021/jp4005218
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发表时间：
2013-03-21
影响因子：
3.3
通讯作者：
Koehler, Juergen
中科院分区：
文献类型：
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作者：
Boehm, Paul S.;Southall, June;Koehler, Juergen
我们重新研究了从Rhodopropylene（Rps.）用于单分子光谱的嗜酸菌。对于本研究，使用相对温和的去污剂（十二烷基-β-D-麦芽糖苷（DDM）代替月桂基二甲胺N-氧化物（LDAO））在去污剂缓冲液中稳定色素-蛋白质复合物。这导致了一个显着减少的分数破碎/解离的RC-LH 1复合物相对于以前的研究，并使我们能够调查一个足够大的样本的个别RC-LH 1复合物。对于大多数的配合物的荧光激发光谱表现出一个狭窄的光谱特征，在光谱的红端。的光谱特性的统计分析产生了密切的相似之处，从Rps的RC-LH 1复合物上获得的结果。沼泽植物，可获得低分辨率X射线结构。基于这种比较，我们得出的结论是，对于这两个物种的RC-LH 1复合物可以描述由相同的结构模型，即，一个整体的椭圆形组装的颜料，具有间隙。
We have revisited the RC-LH1 complex from Rhodopseudomonas (Rps.) acidophila for single-molecule spectroscopy. For the current study the pigment-protein complexes were stabilized in the detergent buffer solution using a relatively mild detergent (dodecyl-beta-D-maltoside (DDM) instead of lauryldimethylamine N-oxide (LDAO)). This leads to a significant reduction of the fraction of broken/dissociated RC-LH1 complexes with respect to previous studies and has allowed us to investigate a sufficiently large sample of individual RC-LH1 complexes. For most of the complexes the fluorescence-excitation spectra exhibit a narrow spectral feature at the red end of the spectrum. Analysis of the statistics of the spectral properties yields a close resemblance with the results obtained on RC-LH1 complexes from Rps. palustris for which a low-resolution X-ray structure is available. Based on this comparison we come to the conclusion that for both species the RC-LH1 complex can be described by the same structural model, that is, an overall elliptical assembly of pigments that features a gap.