Retinol to retinol-binding protein (RBP) is low in obese adults due to elevated apo-RBP.
Retinol to retinol-binding protein (RBP) is low in obese adults due to elevated apo-RBP.
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DOI：
10.3181/0803-rm-94
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发表时间：
2008-10
期刊：
影响因子：
--
通讯作者：
Tanumihardjo SA
中科院分区：
文献类型：
--
作者：
Mills JP;Furr HC;Tanumihardjo SA
血清视黄醇结合蛋白（RBP）浓度升高与肥胖和胰岛素抵抗相关，但尚未报道伴随的视黄醇值。需要对视黄醇进行评估，以区分可能充当脂肪因子的 apo-RBP 和转运维生素 A 的 Holo-RBP。在肥胖成人中确定了血清 RBP、视黄醇、视黄酯、BMI 和胰岛素抵抗指标之间的关系。从肥胖男性和女性 (n = 76) 中采集空腹血液 (≥8 小时) 并进行血液化学分析。视黄醇和视黄酯通过 HPLC 定量，RBP 通过 ELISA 定量。在年龄和性别匹配的非肥胖个体 (n = 41) 中测定 RBP 和视黄醇以进行比较。肥胖受试者的血清 apo-RBP (0.90 ± 0.62 µM) 是非肥胖受试者 (0.44 ± 0.56 µM) 的两倍 (P < 0.001)。肥胖受试者的视黄醇与 RBP 比率（视黄醇：RBP）显着低于非肥胖受试者（0.73 ± 0.13）（0.90 ± 0.22）（P < 0.001），并且两组中 RBP 与视黄醇密切相关（分别为 r = 0.71 和 0.90，P < 0.0001）。在肥胖受试者中，RBP与胰岛素（r = 0.26，P < 0.05）、胰岛素抵抗的稳态模型评估（r = 0.29，P < 0.05）和定量胰岛素敏感性检查指数（r = -0.27，P < 0.05）相关。仅当肥胖和非肥胖受试者合并时，RBP 才与 BMI 相关（r = 0.25，P < 0.01）。血清 RBP 升高部分源自 apo-RBP，与肥胖成人的 BMI 或胰岛素抵抗指标相比，与视黄醇的相关性更强。研究 RBP 在肥胖和胰岛素抵抗中的作用应包括视黄醇，以促进 apo-RBP 和视黄醇：RBP 的测量。在评估降低血清 RBP 的治疗潜力时，考虑对维生素 A 代谢的影响至关重要。
Elevated serum retinol-binding protein (RBP) concentration has been associated with obesity and insulin resistance, but accompanying retinol values have not been reported. Assessment of retinol is required to discriminate between apo-RBP, which may act as an adipokine, and holo-RBP, which transports vitamin A. The relations between serum RBP, retinol, retinyl esters, BMI, and measures of insulin resistance were determined in obese adults. Fasting blood (≥8 h) was collected from obese men and women (n = 76) and blood chemistries were obtained. Retinol and retinyl esters were quantified by HPLC and RBP by ELISA. RBP and retinol were determined in age and sex-matched, nonobese individuals (n = 41) for comparison. Serum apo-RBP was two-fold higher in obese (0.90 ± 0.62 µM) than nonobese subjects (0.44 ± 0.56 µM) (P < 0.001). The retinol to RBP ratio (retinol:RBP) was significantly lower in obese (0.73 ± 0.13) than nonobese subjects (0.90 ± 0.22) (P < 0.001) and RBP was strongly associated with retinol in both groups (r = 0.71 and 0.90, respectively, P < 0.0001). In obese subjects, RBP was associated with insulin (r = 0.26, P < 0.05), homeostatic model assessment of insulin resistance (r = 0.29, P < 0.05), and quantitative insulin sensitivity check index (r = −0.27, P < 0.05). RBP was associated with BMI only when obese and nonobese subjects were combined (r = 0.25, P < 0.01). Elevated serum RBP, derived in part from apo-RBP, was more strongly associated with retinol than with BMI or measures of insulin resistance in obese adults. Investigations into the role of RBP in obesity and insulin resistance should include retinol to facilitate the measurement of apo-RBP and retinol:RBP. When evaluating the therapeutic potential of lowering serum RBP, consideration of the consequences on vitamin A metabolism is paramount.