Environmental Factors Affect Acidobacterial Communities below the Subgroup Level in Grassland and Forest Soils
Environmental Factors Affect Acidobacterial Communities below the Subgroup Level in Grassland and Forest Soils
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DOI：
10.1128/aem.01325-12
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发表时间：
2012-10-01
影响因子：
4.4
通讯作者：
Friedrich, Michael W.
中科院分区：
文献类型：
--
作者：
Naether, Astrid;Foesel, Baerbel U.;Friedrich, Michael W.
在土壤中，酸杆菌平均占所有细菌的20%，具有高度的多样性，并且在现场具有生理活性。然而，它们的个体功能以及与土壤中高等类群的相互作用仍不清楚。在这里，研究了土地利用、土壤性质、植物多样性和土壤纳米动物对德国三个不同地区草原和森林土壤中酸性细菌群落组成的潜在影响。对所有土壤的16S rRNA基因克隆文库的分析表明，草原土壤以Gp6亚类为主，森林土壤以Gp1酸杆菌亚类为主。用16S rRNA基因指纹分析方法(T-RFLP和变性梯度凝胶电泳法)分析了草原和森林土壤中的大量位点(n=57)，结果表明，酸杆菌的多样性不仅在草原和森林土壤之间存在差异，而且在三个不同地区之间也存在差异。土壤性质，如pH、有机碳、全氮、C/N比、磷、硝酸盐、氨氮、土壤水分、土壤温度和土壤呼吸作用，通过指纹分析对群落组成有影响。然而，不同亚群的T-RFs与环境参数的相关性差异很大，不同的Gp1T-RFs与含氮量呈正相关或负相关。只有通过克隆序列的分析，才能发现酸杆菌亚群(即亚群内的个体种群)与土壤纳米动物和维管植物多样性之间的新的显著相关性。因此，为了检测环境参数与酸杆菌的新的相互关系，必须考虑亚组内的个体种群。
In soil, Acidobacteria constitute on average 20% of all bacteria, are highly diverse, and are physiologically active in situ. However, their individual functions and interactions with higher taxa in soil are still unknown. Here, potential effects of land use, soil properties, plant diversity, and soil nanofauna on acidobacterial community composition were studied by cultivation-independent methods in grassland and forest soils from three different regions in Germany. The analysis of 16S rRNA gene clone libraries representing all studied soils revealed that grassland soils were dominated by subgroup Gp6 and forest soils by subgroup Gp1 Acidobacteria. The analysis of a large number of sites (n = 57) by 16S rRNA gene fingerprinting methods (terminal restriction fragment length polymorphism [T-RFLP] and denaturing gradient gel electrophoresis [DGGE]) showed that Acidobacteria diversities differed between grassland and forest soils but also among the three different regions. Edaphic properties, such as pH, organic carbon, total nitrogen, C/N ratio, phosphorus, nitrate, ammonium, soil moisture, soil temperature, and soil respiration, had an impact on community composition as assessed by fingerprinting. However, interrelations with environmental parameters among subgroup terminal restriction fragments (T-RFs) differed significantly, e.g., different Gp1 T-RFs correlated positively or negatively with nitrogen content. Novel significant correlations of Acidobacteria subpopulations (i.e., individual populations within subgroups) with soil nanofauna and vascular plant diversity were revealed only by analysis of clone sequences. Thus, for detecting novel interrelations of environmental parameters with Acidobacteria, individual populations within subgroups have to be considered.