Transcription factors Sp8 and Sp9 regulate the development of caudal ganglionic eminence-derived cortical interneurons
Transcription factors Sp8 and Sp9 regulate the development of caudal ganglionic eminence-derived cortical interneurons
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转录因子 Sp8 和 Sp9 调节尾部神经节隆起衍生的皮质中间神经元的发育
DOI：
10.1002/cne.24712
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发表时间：
2019
影响因子：
2.5
通讯作者：
Yang Zhengang
中科院分区：
文献类型：
--
作者：
Wei Song;Du Heng;Li Zhenmeiyu;Tao Guangxu;Xu Zhejun;Song Xiaolei;Shang Zicong;Su Zihao;Chen Haotian;Wen Yan;Liu Guoping;You Yan;Zhang Zhuangzhi;Yang Zhengang
Cortical interneurons are derived from the subcortical medial ganglionic eminence (MGE), caudal ganglionic eminence (CGE) and preoptic area (POA). CGE‐derived cortical interneurons, which comprise around 30% of all cortical interneurons, mainly express Htr3a, calretinin (CR), Reelin (RELN) and vasoactive intestinal polypeptide (VIP). In the present study, we show that transcription factorsSp8andSp9are co‐expressed in the subventricular zone (SVZ) of the dorsal CGE. Conditional knockout ofSp8/9using theGsx2‐Cretransgenic line results in severe loss of CGE‐derived cortical interneurons. We observed migration defects ofSp8/9double mutant CGE‐derived cortical interneurons as they had longer leading processes than controls and they ectopically accumulated in the CGE.Dlx5/6‐CIEconditional deletion ofSp8/9also leads to a significant reduction in the CGE‐derived cortical interneurons. We provide evidence thatSp8/9coordinately regulate CGE‐derived cortical interneuron development in part through repressing the expression ofPak3,Robo1, andSlit1. Finally, we show thatCxcl14, a member of the CXC chemokine family, is mainly expressed in CGE‐derived interneurons in cortical layers I and II, and its expression is critically dependent on SP8.
