Immunomagnetic reduction assay using high-Tc superconducting-quantum-interference-device-based magnetosusceptometry
Immunomagnetic reduction assay using high-Tc superconducting-quantum-interference-device-based magnetosusceptometry
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DOI：
10.1063/1.3340861
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发表时间：
2010-04-01
影响因子：
3.2
通讯作者：
Yang, Hong-Chang
中科院分区：
文献类型：
--
作者：
Chieh, J. J.;Yang, Shieh-Yueh;Yang, Hong-Chang
通过免疫磁性还原分析，可以借助于生物功能化的磁性纳米颗粒来定量检测生物分子，所述磁性纳米颗粒用作特定生物分子的标记物。为了实现超高灵敏度的生物分子检测，超导量子干涉器件（SQUID）是一种很有前途的候选传感器，可以作为与被检测生物分子浓度相关的磁信号的传感器。在过去，我们开发了一个单通道SQUID为基础的磁共振测量。为了提高检测通量，发展了基于SQUID的多通道磁共振技术。介绍了四通道SQUID磁敏仪的设计和工作原理。利用扫描技术，四个样品可以同时记录到基于SQUID的磁共振测量中。值得注意的是，只有单一的SQUID磁强计中使用的磁电阻测量。研究了四通道SQUID磁共振技术在生物分子检测中的精密度和灵敏度。(C)2010年美国物理学会。[doi：10.1063/1.3340861]
Via immunomagnetic reduction assay, biomolecules can be quantitatively detected with aid of biofunctionalized magnetic nanoparticles, which are used as labeling markers for specific biomolecules. To achieve ultra-high sensitivity in detecting biomolecules, superconducting quantum interference device (SQUID) is a promising candidate to act as a sensor to the magnetic signal related to the concentration of detected biomolecules. In the past, we developed a single channel SQUID-based magnetosusceptometry. In order to increase the detection through-put, multichannel SQUID-based magnetosusceptometry is developed. In this work, the design and working principle of four-channel SQUID-based magnetosusceptometry are introduced. Using utilizing scanning technology, four samples can be simultaneously logged into the SQUID-based magnetosusceptometry. Notably, only single SQUID magnetometer is used in the magnetosusceptometry. The precision and sensitivity in detecting biomolecules using the four-channel SQUID-based magnetosusceptometry are investigated. (C) 2010 American Institute of Physics. [doi:10.1063/1.3340861]