Increased Kappa/Lambda Hybrid Antibody in Serum Is a Novel Biomarker Related to Disease Activity and Inflammation in Rheumatoid Arthritis.
Increased Kappa/Lambda Hybrid Antibody in Serum Is a Novel Biomarker Related to Disease Activity and Inflammation in Rheumatoid Arthritis.
复制标题
血清中增加的 Kappa/Lambda 混合抗体是与类风湿关节炎疾病活动和炎症相关的新型生物标志物
DOI：
10.1155/2016/2953072
复制
发表时间：
2016
影响因子：
4.6
通讯作者：
Li J
中科院分区：
文献类型：
--
作者：
Yi L;Hao M;Lu T;Lin G;Chen L;Gao M;Fan G;Zhang D;Wang G;Yang X;Li Y;Zhang K;Zhang R;Han Y;Wang L;Li J
最近报道了正常人血清中的κ/λ杂合抗体，但其临床意义尚未被探索。类风湿性关节炎（RA）是主要的关节疾病之一，RA的早期诊断和治疗仍然是一个挑战。在这里，我们开发了一种双夹心酶联免疫吸附测定系统，用于量化 RA 患者、骨关节炎 (OA) 患者和健康对照 (HC) 的相对血清 κ/λ 混合抗体水平，以评估其作为早期疾病和临床活性的血清学生物标志物的潜在用途，并初步研究其在 RA 中的免疫调节作用。令人惊讶的是，我们发现 κ/λ 杂合抗体在早期和确诊的 RA 中均显着增加。 RA血清中κ/λ杂合抗体水平与临床指标、炎症标志物显着相关。进一步分析显示κ/λ混合抗体水平与28关节疾病活动评分（DAS28）呈正相关。总之，首次鉴定出 RA 血清中的 κ/λ 杂合抗体。高水平的 κ/λ 混合抗体可能是区分早期 RA 与 OA 和 HC 的有用工具。我们建议使用 κ/λ 混合抗体作为疾病活动性的标志物。 κ/λ 杂合抗体的增加与 RA 的炎症状况相关。
The κ/λ hybrid antibodies in normal human serum were reported recently, but their clinical relevance has not yet been explored. Rheumatoid arthritis (RA) is one of the major joint diseases, and the early diagnosis and treatment of RA remain a challenge. Here, we developed a double-sandwich enzyme-linked immunosorbent assay system to quantify relative serum κ/λ hybrid antibody levels in RA patients, osteoarthritis (OA) patients, and healthy controls (HC) in order to assess their potential use as a serological biomarker of early disease and clinical activity and to preliminarily investigate their immunomodulatory roles in RA. Surprisingly, we found that κ/λ hybrid antibody was markedly increased in both early and established RA. Serum κ/λ hybrid antibody levels were significantly correlated with clinical indexes and inflammatory markers in RA. Further analysis showed a positive correlation between κ/λ hybrid antibody levels and the 28-joint disease activity score (DAS28). In conclusion, serum κ/λ hybrid antibodies in RA were identified for the first time. High levels of κ/λ hybrid antibody may be a useful tool in distinguishing early RA from OA and HC. We suggest κ/λ hybrid antibody as a marker for disease activity. The increased κ/λ hybrid antibodies were associated with inflammatory conditions in RA.