Regulatory roles of fibronectin and integrin α5 in reorganization of the actin cytoskeleton and completion of adipogenesis.
Regulatory roles of fibronectin and integrin α5 in reorganization of the actin cytoskeleton and completion of adipogenesis.
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DOI：
10.1091/mbc.e21-12-0609
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发表时间：
2022-08-01
影响因子：
3.3
通讯作者：
Nobusue, Hiroyuki
中科院分区：
文献类型：
--
作者：
Uetaki, Megumi;Onishi, Nobuyuki;Oki, Yoshinao;Shimizu, Takatsune;Sugihara, Eiji;Sampetrean, Oltea;Watanabe, Takashi;Yanagi, Hisano;Suda, Kiyoshi;Fujii, Hiroya;Kano, Koichiro;Saya, Hideyuki;Nobusue, Hiroyuki
细胞分化的特征在于细胞形态的变化，其在很大程度上由肌动蛋白动力学决定。我们以前表明，解聚的肌动蛋白细胞骨架触发分化的前脂肪细胞成成熟的脂肪细胞作为结果的巨核细胞白血病1（MKL 1）的转录辅激活因子活性的抑制。细胞外基质（ECM）通过与整合素相互作用影响细胞形态，ECM的重组与细胞分化有关。在这里，我们表明，肌动蛋白动力学和ECM重排之间的相互作用在脂肪细胞分化中起着关键作用。我们发现，解聚的肌动蛋白细胞骨架的破坏和降解的纤维状纤连蛋白（FN）结构在细胞表面诱导后，在培养的前脂肪细胞的脂肪形成。FN基质抑制肌动蛋白细胞骨架重组为脂肪细胞的模式特征和终末脂肪细胞分化，这些抑制作用可通过敲低整合素α5（ITGα5）来克服。过氧化物酶体增殖物激活受体γ是脂肪细胞分化过程中FN下调所必需的，而MKL 1是ITGα5表达所必需的。我们的研究结果表明，FN-ITGα5相互作用的细胞自主下调有助于肌动蛋白细胞骨架的重组和脂肪细胞分化的完成。
Cellular differentiation is characterized by changes in cell morphology that are largely determined by actin dynamics. We previously showed that depolymerization of the actin cytoskeleton triggers the differentiation of preadipocytes into mature adipocytes as a result of inhibition of the transcriptional coactivator activity of megakaryoblastic leukemia 1 (MKL1). The extracellular matrix (ECM) influences cell morphology via interaction with integrins, and reorganization of the ECM is associated with cell differentiation. Here we show that interaction between actin dynamics and ECM rearrangement plays a key role in adipocyte differentiation. We found that depolymerization of the actin cytoskeleton precedes disruption and degradation of fibrillar fibronectin (FN) structures at the cell surface after the induction of adipogenesis in cultured preadipocytes. A FN matrix suppressed both reorganization of the actin cytoskeleton into the pattern characteristic of adipocytes and terminal adipocyte differentiation, and these inhibitory effects were overcome by knockdown of integrin α5 (ITGα5). Peroxisome proliferator–activated receptor γ was required for down-regulation of FN during adipocyte differentiation, and MKL1 was necessary for the expression of ITGα5. Our findings suggest that cell-autonomous down-regulation of FN-ITGα5 interaction contributes to reorganization of the actin cytoskeleton and completion of adipocyte differentiation.
DOI：
10.1111/gtc.12814
发表时间：
2020-12
期刊：
Genes to cells : devoted to molecular & cellular mechanisms
影响因子：
--
作者：
Hagiwara R;Oki Y;Matsumaru T;Ibayashi S;Kano K
通讯作者：
Kano K
Rho/MRTF-A 诱导的整合素表达调节分化多能间充质干细胞中的血管生成
影响因子：
4.3
作者：
Zhang R;Wang N;Zhang M;Zhang LN;Guo ZX;Luo XG;Zhou H;He HP;Zhang TC
通讯作者：
Zhang TC