Robust and hydrophilic polymeric films with honeycomb pattern and their cell scaffold applications
Robust and hydrophilic polymeric films with honeycomb pattern and their cell scaffold applications
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具有蜂窝图案的坚固亲水聚合物薄膜及其细胞支架应用
DOI：
10.1039/b820279f
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发表时间：
2009-01-01
影响因子：
--
通讯作者：
Ma, Zhi
中科院分区：
文献类型：
--
作者：
Li, Lei;Chen, Caikang;Ma, Zhi
以市售嵌段共聚物聚苯乙烯-b-聚丁二烯-b-聚苯乙烯（SBS）为原料，通过在高湿条件下蒸发不同浓度的SBS/二硫化碳溶液，成功制备了微图纹聚合物薄膜。研究了溶液浓度对孔径和排列的影响。随着溶液浓度的增加，规则的孔隙排列由于约定性减弱而变得杂乱无章。当浓度大于60 mg mL−1时，出现双峰型大小分布。将聚合物薄膜暴露在深紫外光（波长= 254 nm）下，通过衰减全反射傅立叶变换红外光电子能谱和x射线光电子能谱进行监测，实现了光化学交联。交联1h后，膜的耐溶剂性、热稳定性和表面润湿性均显著提高。交联的蜂窝结构薄膜耐各种有机溶剂，热稳定性高达350°C，与未交联的薄膜相比，增加了250多K。光化学过程的另一个好处是在薄膜表面形成极性基团，将表面润湿性从疏水性转变为亲水性，微图案表面增强了润湿性。所得膜无细胞毒性，因此适合作为细胞支架。研究发现，具有3µm均匀孔径的亲水性微图案聚合物薄膜有利于细胞的附着和增殖。
Starting from a commercially available block copolymer, polystyrene-b-polybutadiene-b-polystyrene (SBS), micro-patterned polymer films were successfully prepared by evaporating SBS/carbon disulfide solutions with different concentrations under high humidity. The influence of solution concentration on the pore size and array was investigated in detail. With the increase of solution concentration, regular pore arrays turned into random because of weakened convention. Once the concentration was more than 60 mg mL−1, a bimodal size distribution appeared. Photo-chemical cross-linking was achieved by exposing the polymer films in deep UV light (wavelength = 254 nm), monitored by attenuated total reflective Fourier transform infrared and X-ray photoelectron spectroscopy. After 1 h cross-linking, either solvent resistance or thermal stability or surface wettability of the films was significantly improved. The cross-linked honeycomb structured films became resistant to a wide range of organic solvents and thermally stable up to 350 °C, an increase of more than 250 K as compared to the un-crosslinked films. Another beneficial effect of the photo-chemical process was the formation of polar groups on the film surface, changing surface wettability from hydrophobicity to hydrophilicity, enhanced by the micro-patterned surface. The resultant films were non-cytotoxic and hence suitable as cell scaffolds. It was found that hydrophilic, micro-patterned polymer films with a uniform pore size of 3 µm facilitated cell attachment and proliferation.