Pressurized Liquid Extraction as an Alternative Process To Obtain Antiviral Agents from the Edible Microalga Chlorella vulgaris
Pressurized Liquid Extraction as an Alternative Process To Obtain Antiviral Agents from the Edible Microalga Chlorella vulgaris
复制标题
DOI：
10.1021/jf100369h
复制
发表时间：
2010-08-11
影响因子：
6.1
通讯作者：
Senorans, Francisco J.
中科院分区：
文献类型：
--
作者：
Santoyo, Susana;Plaza, Merichel;Senorans, Francisco J.
研究了食用小球藻加压提取物（PLE）（丙酮、乙醇和水）对单纯疱疹病毒1型（HSV-1）的抗病毒活性。尽管在加入病毒前用75 μ g/mL水和乙醇提取物预处理Vero细胞可抑制70%的病毒感染，但测试的提取物均未显示出对病毒的细胞外直接杀病毒活性。此外，水和乙醇提取物能够显著抑制体外病毒复制，显示IC 50%值分别为61.05和80.23 μ g/mL。为了鉴定在水提取物中发现的负责抗病毒活性的化合物的类型，分离多糖级分。发现这种活性与多糖相关，因为富含多糖的级分（46%浓缩）显示出比完全水提取物更高的抗病毒活性。当作为预处理添加时，浓度为75 μ g/mL的该组分抑制90%的病毒感染，并且对于细胞内病毒复制显示出33.93 μ g/mL的IC 50%值。乙醇提取物的GC-MS表征表明，该提取物的抗病毒活性可能部分与植醇的存在有关，虽然其他化合物可能参与这种活性。
The antiviral properties of pressurized liquid extracts (PLE) (acetone, ethanol, and water) obtained from the edible microalga Chlorella vulgaris were evaluated against herpes simplex virus type 1 (HSV-1). None of the extracts tested showed extracellular direct virucidal activity against the virus, although a pretreatment of Vero cells with 75 mu g/mL of water and ethanol extracts before virus addition inhibited 70% of the virus infection. Moreover, water and ethanol extracts were able to significantly inhibit the in vitro virus replication, showing IC50% values of 61.05 and 80.23 mu g/mL respectively. To identify the type of compounds responsible for the antiviral activity found in the water extract, the polysaccharide fraction was isolated. This activity was found to correlate with polysaccharides, because the polysaccharide-rich fraction (46% concentrated) showed higher antiviral activity than the complete water extract. A concentration of 75 mu g/mL of this fraction inhibited 90% virus infection when added as a pretreatment and showed an IC50% value of 33.93 mu g/mL for intracellular virus replication. GC-MS characterization of the ethanol extract showed that the antiviral activity of this extract could be partially related with the presence of phytol, although other compounds could be involved in this activity.