Evaluation of a partial optic nerve crush model in rats.
Evaluation of a partial optic nerve crush model in rats.
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大鼠部分视神经挤压模型的评价
DOI：
10.3892/etm.2012.619
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发表时间：
2012-09
影响因子：
2.7
通讯作者：
Zhong YS
中科院分区：
文献类型：
--
作者：
Tan HB;Shen X;Cheng Y;Jiao Q;Yang ZJ;Zhong YS
本研究旨在确定大鼠部分视神经损伤（PONC）模型是否是研究视神经保护和再生的有效和可靠的模型。用1，1 ′-双十八烷基-3，3，3 ′，3 ′-四甲基吲哚羰花青高氯酸盐（DiI）逆行标记成年SD大鼠视网膜神经节细胞（RGCs; n=3）的双侧上级丘（SC）和单侧RGCs（n=3），并与双侧和单侧SC逆行标记的RGCs进行比较。另取40只成年SD大鼠，在双侧SC逆行DiI标记RGCs后3d，于右侧视乳头后1 mm处用无创血管夹（40 g功率）夹压5、10、30 sec（各10只），并设假手术对照组（n = 10）作为对照。所有40只大鼠的视网膜通过四个径向切口变平，玻璃体侧向上安装在明胶涂覆的载玻片上，并且在三天后在视网膜半径的1/6、3/6和5/6的三个不同偏心率下在每个视网膜象限的四个不同区域中计数标记的RGC的数量。双侧SC逆行注射DiI标记了大多数正常RGC，而单侧SC注射仅标记了一小部分RGC;大多数RGC未被标记。在轻度挤压（5秒）损伤组中，双侧SC逆行注射DiI标记了大部分RGC。视网膜半径1/6、3/6、5/6处的RGC密度与假手术对照组相应区域的RGC密度比较，差异无统计学意义（P=0.734、0.461、0.273）。在中度挤压伤（10秒）组中，标记的RGC的数量显著低于假手术对照组，并且在视网膜半径的1/6、3/6、5/6处的RGC密度显著低于假手术对照组中相应视网膜半径处的RGC密度（P<0.001）。在重度挤压伤（30秒）组中，标记的RGC数量显著减少，在视网膜半径的5/6区域未观察到标记的RGC。假手术对照组视网膜半径1/6和3/6处的RGC密度明显低于相应区域的RGC密度（P<0.001）。与轻、重度视神经挤压伤模型相比，中度视神经挤压伤模型更适合于视神经损伤与再生的研究。
This study was performed to determine whether a partial optic nerve crush (PONC) model in rats is effective and reliable for the study of optic nerve protection and regeneration. Bilateral superior colliculus (SC) retrograde 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI) labeling of retinal ganglion cells (RGCs; n=3) and unilateral SC retrograde labeling of RGCs (n=3) were performed in adult Sprague-Dawley (SD) rats and the results were compared with the bilateral and unilateral SC retrograde-labeled RGCs. Another 40 adult SD rats, three days after bilateral SC retrograde DiI labeling of RGCs underwent crushing with a non-invasive vascular clip (40 gram power) 1 mm behind the right optic nerve head for 5, 10 and 30 sec (n=10 each), and a sham-operated control group (n=10) was used as a control. The retinas of all 40 rats were flattened by four radial cuts, mounted vitreal side-up on gelatin-coated slides, and the number of labeled RGCs was counted in four distinct regions per retinal quadrant at three different eccentricities of 1/6, 3/6 and 5/6 of the retinal radius three days later. Bilateral SC retrograde DiI injection labeled the majority of normal RGCs, while unilateral SC injections only labeled a small part of the RGCs; the majority of RGCs were not labeled. In the mild crush (5 sec) injury group, the bilateral SC retrograde DiI injection labeled the majority of RGCs. The RGC densities at 1/6, 3/6 and 5/6 of the retinal radius showed no significant difference compared with the RGC densities at the corresponding region of the retinal radius in the sham-operated control group (P=0.734, 0.461, 0.273, respectively). In the moderate crush injury (10 sec) group, the number of labeled RGCs was significantly lower compared to that of the sham-operated control group, and the RGC densities at 1/6, 3/6, 5/6 of the retinal radius were significantly lower compared to the RGC densities at the corresponding retinal radius in the sham-operated control group (P<0.001). In the severe crush injury (30 sec) group the number of labeled RGCs was significantly decreased, and the labeled RGCs were not observed in the region at 5/6 of the retinal radius. The RGC densities at 1/6 and 3/6 of the retinal radius were significantly lower compared to the RGC densities at the corresponding retinal radius region in the sham-operated control group (P<0.001). Compared with the mild and severe optic nerve crush injury models, the moderate crush injury model is more suitable for the study of optic nerve damage and regeneration.
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影响因子：
64.8
作者：
FORRESTER, J;PETERS, A
通讯作者：
PETERS, A
影响因子：
3.4
作者：
Yu, Saiyuu;Tanabe, Teruyo;Yoshimura, Nagahisa
通讯作者：
Yoshimura, Nagahisa
影响因子：
3.5
作者：
Kretz, A;Happold, CJ;Isenmann, S
通讯作者：
Isenmann, S
影响因子：
14.5
作者：
Lingor, Paul;Tonges, Lars;Baehr, Mathias
通讯作者：
Baehr, Mathias
影响因子：
3.4
作者：
Thaler, Sebastian;Fiedorowicz, Michal;Schuettauf, Frank
通讯作者：
Schuettauf, Frank