Protective and pathological activity in serum of mice developing resistance to Plasmodium berghei infection
Protective and pathological activity in serum of mice developing resistance to Plasmodium berghei infection
复制标题
对伯氏疟原虫感染产生抵抗力的小鼠血清中的保护性和病理活性
DOI：
10.1111/j.1365-3024.1989.tb00678.x
复制
发表时间：
1989
影响因子：
2.2
通讯作者：
W. Eling
中科院分区：
文献类型：
--
作者：
T. Schetters;J. Breda;C. Hermsen;J. Curfs;W. Eling
总结已经在体内和体外分析了来自使用化疗治疗对伯氏疟原虫感染产生抗性的小鼠的血清。在免疫期间，产生了可以通过血清转移的病理性和保护性活性。病理活动，这是定义为破坏红细胞在正常受体小鼠，产生早期的免疫程序，在第21天达到高峰，并降低到检测不到的水平，由第35天。供体小鼠再次感染后，血清中再次出现病理活性，并维持至少56天。对转移的血清样本进行分析，结果显示存在抗红细胞抗体（ELISA），但未发现与体内抗红细胞效应的相关性。血清样本的抗红细胞效应通过诱导网织红细胞增多间接增加了受体小鼠的寄生虫血症。仅在免疫程序第61天后采集的动物样本中检测到血清样本的保护作用。这种净保护作用反映在用伯氏疟原虫感染的红细胞攻击受体小鼠后7天寄生虫血症减少。血清的保护活性与抗红细胞抗体的高滴度相关。抗红细胞抗体滴度与异源红细胞滴度以及血清样本的总免疫球蛋白含量密切相关，表明淋巴细胞的多克隆活化。除IgGl外，所有（亚）类在免疫程序期间均升高，其中IgG3是丰富的。获得免疫后，这些免疫球蛋白水平保持高水平，并且血清中IgGl的相对量恢复。
Summary The serum from mice developing resistance against Plasmodium berghei infection using chemotherapeutic treatment has been analysed in vivo and in vitro. During the immunization period pathological as well as protective activities which could be transferred by serum were generated. The pathological activity, which was defined as destruction of erythrocytes in normal recipient mice, was generated early in the immunization procedure, peaked at day 21, and decreased to undetectable levels by day 35. After reinfection of the donor mice the pathological activity reappeared in the serum, and was maintained for at least 56 days. Analysis of the transferred serum samples showed the presence of anti‐erythrocyte antibodies (ELISA), but no correlation with the in‐vivo anti‐erythrocyte effect could be found. The anti‐erythrocyte effect of the serum samples indirectly increased the parasitaemia in the recipient mice through the induction of reticulocytosis. The protective effect of the serum samples could only be detected in samples taken from animals beyond day 61 of the immunization procedure. This net protective effect was reflected in a decreased parasitaemia at 7 days after challenge of the recipient mice with P. berghei infected erythrocytes. The protective activity of the serum was correlated with high titres of anti‐erythrocyte antibodies. Anti‐erythrocyte antibody titres were strongly correlated with titres against heterologous red blood cells as well as total immunoglobulin content of the serum samples, indicative of polyclonal activation of lymphocytes. Except for IgGl, all (sub‐) classes were elevated during the immunization procedure, of which IgG3 was abundant. After immunity was obtained these immunoglobulin levels remained high, and the relative amount of IgGl in the serum was restored.