Meiotic prophase arrest with failure of chromosome synapsis in mice deficient for Dmc1, a germline-specific RecA homolog
Meiotic prophase arrest with failure of chromosome synapsis in mice deficient for Dmc1, a germline-specific RecA homolog
复制标题
DOI：
10.1016/s1097-2765(00)80069-6
复制
发表时间：
1998-04-01
期刊：
影响因子：
16
通讯作者：
Schimenti, JC
中科院分区：
文献类型：
--
作者：
Pittman, DL;Cobb, J;Schimenti, JC
DMC 1是首次在酵母中发现的减数分裂特异性基因，其编码与RecA同源的蛋白质，并且可能是重组结节的组分。酵母dmc 1突变体在交换和联会复合体（SC）的形成方面有缺陷，并且在减数分裂I的后期前期停滞。我们已经在小鼠中产生了Dmc 1基因的无效突变，并且表明纯合突变的雄性和雌性都是不育的，在第一次减数分裂前期配子发生停滞。突变体精母细胞中的染色体不能突触，尽管形成的轴向元素的前体SC。在Dmc 1缺陷小鼠和酵母的表型的强烈相似性表明，减数分裂机制已高度保守，通过进化。
DMC1 is a meiosis-specific gene first discovered in yeast that encodes a protein with homology to RecA and may be a component of recombination nodules. Yeast dmc1 mutants are defective in crossing over and synaptonemal complex (SC) formation, and arrest in late prophase of meiosis I, We have generated a null mutation in the Dmc1 gene in mice and show that homozygous mutant males and females are sterile with arrest of gametogenesis in the first meiotic prophase. Chromosomes in mutant spermatocytes fail to synapse, despite the formation of axial elements that are the precursor to the SC. The strong similarity of phenotypes in Dmc1-deficient mice and yeast suggests that meiotic mechanisms have been highly conserved through evolution.