EFFECT OF STORAGE MEDIA ON SUBSEQUENT WATER-LOSS AND REGAIN BY HUMAN AND BOVINE DENTIN AND ON MECHANICAL-PROPERTIES OF HUMAN DENTIN IN-VITRO
EFFECT OF STORAGE MEDIA ON SUBSEQUENT WATER-LOSS AND REGAIN BY HUMAN AND BOVINE DENTIN AND ON MECHANICAL-PROPERTIES OF HUMAN DENTIN IN-VITRO
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DOI：
10.1016/0003-9969(94)90005-1
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发表时间：
1994-09-01
影响因子：
3
通讯作者：
HOOD, JAA
中科院分区：
文献类型：
--
作者：
JAMESON, MW;TIDMARSH, BG;HOOD, JAA
本研究旨在确定储存介质和时间是否会影响牙本质脱水时水分流失的任何变化。还将制备的棒的储存效果与整个牙齿的储存进行了比较。将制备的牛牙本质条在去离子水、磷酸盐缓冲盐水或中性缓冲福尔马林中储存3天或12周，并且在制备牙本质条之前将整个牙齿在中性缓冲福尔马林中储存12周。从储存中取出样本，通过重量分析测定的脱水失重直接等同于水分损失。对在去离子水或中性缓冲福尔马林中储存3天的人牙本质棒进行拉伸和三点弯曲测试。对结果进行方差分析和邓肯新的多范围检验。在22.3摄氏度和53.2%相对湿度下，牙本质蒸发的重量损失是快速和非线性的，7天后牛牙本质达到5.05 +/- 0.55%，而人牙本质为3.33 +/- 0.63%。脱水失水不受3天的存储标本在不同的媒体或12周的存储整个牙齿，但受影响时，准备标本存储12周。不同保存介质对牙本质的强度和韧性无显著影响（p > 0.05）。人牙本质中的水分流失与样本的收缩有关，但这些尺寸变化在再水化后完全恢复。建议将牙齿储存在中性缓冲福尔马林中，然后将制备的标本储存时间缩短，以尽量减少拔牙后牙本质的变化。
This study was designed to determine whether storage media and time affect any changes in water loss with dehydration of dentine. The effect of storage of prepared bars was also compared with storage of whole teeth. Prepared bovine dentine bars were stored for 3 days or 12 weeks in deionized water, phosphate-buffered saline or neutral-buffered formalin and whole teeth were stored in neutral-buffered formalin for 12 weeks before the preparation of dentine bars. Specimens were removed from storage and weight loss with dehydration, determined gravimetrically, was equated directly to water loss. Tensile and three-point bend tests were made on human dentine bars that had been stored in deionized water or neutral-buffered formalin for 3 days. Results were subjected to analysis of variance nd Duncan's new multiple-range test. Weight loss by evaporation from dentine at 22.3 degrees C and 53.2% relative humidity was rapid and non-linear, reaching 5.05 +/- 0.55% for bovine dentine compared with 3.33 +/- 0.63% for human dentine after 7 days. Water loss with dehydration was not affected by 3-day storage of specimens in different media or by 12-week storage of whole teeth, but was affected when prepared specimens were stored for 12 weeks. Different storage media had no significant effect (p > 0.05) on the strength or toughness of human dentine. Water loss in human dentine was associated with shrinkage of specimens, but these dimensional changes were fully recovered with rehydration. Storage of teeth in neutral-buffered formalin followed by minimal storage time for prepared specimens is recommended to minimize changes in dentine after extraction.