Neighborhood watch: tools for defining locale-dependent subproteomes and their contextual signaling activities.
Neighborhood watch: tools for defining locale-dependent subproteomes and their contextual signaling activities.
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DOI：
10.1039/d0cb00041h
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发表时间：
2020-06-01
影响因子：
4.1
通讯作者：
Aye Y
中科院分区：
文献类型：
--
作者：
Long MJC;Zhao Y;Aye Y
Transient associations between numerous organelles—e.g., the endoplasmic reticulum and the mitochondria—forge highly-coordinated, particular environments essential for cross-compartment information flow. Our perspective summarizes chemical–biology tools that have enabled identifying proteins present within these itinerant communities against the bulk proteome, even when a particular protein's presence is fleeting/substoichiometric. However, proteins resident at these ephemeral junctions also experience transitory changes to their interactomes, small-molecule signalomes, and, importantly, functions. Thus, a thorough census of sub-organellar communities necessitates functionally probing context-dependent signaling properties of individual protein-players. Our perspective accordingly further discusses how repurposing of existing tools could allow us to glean a functional understanding of protein-specific signaling activities altered as a result of organelles pulling together. Collectively, our perspective strives to usher new chemical–biology techniques that could, in turn, open doors to modulate functions of specific subproteomes/organellar junctions underlying the nuanced regulatory subsystem broadly termed as contactology. We discuss chemical-genetic means to mine locale-specific proteins of functional relevance and whose behaviors are pharmacologically malleable.
