ALTERATIONS IN PHOSPHOLIPID COMPOSITION OF ESCHERICHIA COLI B DURING GROWTH AT DIFFERENT TEMPERATURES
ALTERATIONS IN PHOSPHOLIPID COMPOSITION OF ESCHERICHIA COLI B DURING GROWTH AT DIFFERENT TEMPERATURES
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DOI：
10.1128/jb.100.3.1342-1349.1969
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发表时间：
1969-01-01
影响因子：
3.2
通讯作者：
DESIERVO, AJ
中科院分区：
文献类型：
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作者：
DESIERVO, AJ
大肠杆菌b的主要磷脂类，磷脂酰乙醇胺，心磷脂和磷脂酰甘油，在生长周期的不同阶段进行了定量。有机体在27℃和37℃下孵育，每克细胞（干重）的总脂质磷含量和单个磷脂的相对百分比都有显著差异。在37℃时，脂质磷的总量在整个生长周期中显著下降。然而，在27℃时，总脂质磷积累。大肠杆菌的三个主要磷脂类别的模式呈现复杂的定量变化。此外，一些基于甘油与磷酸盐摩尔比的证据表明，在e的生长后期，磷脂酰甘油磷酸取代了磷脂酰甘油。磷脂和脂肪酸模式的比较分析导致了一个假设，试图解释一些报道的大肠杆菌脂质组成的变化。在不同的生长条件下。
The major phospholipid classes ofEscherichia coliB, phosphatidyl ethanolamine, cardiolipin, and phosphatidyl glycerol, were quantitated at different stages of the growth cycle. The organisms were incubated at both 27 and 37 C. Significant differences were observed both in the amounts of total lipid phosphorus per gram (dry weight) of cells and in the relative percentages of the individual phospholipids. At 37 C the total amount of lipid phosphorus decreased significantly throughout the growth cycle. However, at 27 C total lipid phosphorus accumulated. The patterns of the three major phospholipid classes ofEscherichia coliexhibited complex quantitative changes. In addition, some evidence based on glycerol to phosphate molar ratios indicated that phosphatidyl glycerolphosphate replaced phosphatidyl glycerol during the late growth stages ofE. coliB when grown at 27 C. A comparative analysis of phospholipid and fatty acid patterns led to a hypothesis attempting to explain some reported variations in the lipid composition ofE. coliunder different conditions of growth.