Vaccinia virus infection suppresses the cell microRNA machinery
Vaccinia virus infection suppresses the cell microRNA machinery
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DOI：
10.1007/s00705-012-1366-z
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发表时间：
2012-09-01
影响因子：
2.7
通讯作者：
Shemer-Avni, Yonat
中科院分区：
文献类型：
--
作者：
Grinberg, Moran;Gilad, Shlomit;Shemer-Avni, Yonat
MicroRNAs是通过转录后抑制调控基因表达的关键角色。它们参与生理过程，因此它们的失调可能有助于疾病的发展和癌症的进展。病毒编码的microrna和宿主源microrna在控制病毒生命周期和免疫方面发挥着重要作用。本研究的目的是确定牛痘病毒（VACV）感染对宿主编码microrna表达的影响。在许多细胞类型的VACV感染后24小时内，观察到感染细胞中大多数microrna的明显普遍抑制。我们证明这种抑制与Dicer1酶的表达减少有关，Dicer1酶是microrna产生的关键酶。
MicroRNAs are key players in the regulation of gene expression by posttranscriptional suppression. They are involved in physiological processes, and thus their deregulation may contribute to the development of diseases and progression of cancer. Virus-encoded microRNAs and microRNAs of host origin play an important role in controlling the virus life cycle and immunity. The aim of this study was to determine the effect of vaccinia virus (VACV) infection on the expression of host-encoded microRNAs. A marked general suppression of most microRNAs in the infected cells was observed within 24 hours after VACV infection of a number of cell types. We demonstrate that this suppression was associated with abrogation of expression of the Dicer1 enzyme, which is a key enzyme in the generation of microRNAs.