Absence of cytotoxic antibody to human immunodeficiency virus-infected cells in humans and its induction in animals after infection or immunization with purified envelope glycoprotein gp120.
Absence of cytotoxic antibody to human immunodeficiency virus-infected cells in humans and its induction in animals after infection or immunization with purified envelope glycoprotein gp120.
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人类感染人类免疫缺陷病毒的细胞不存在细胞毒性抗体，而动物感染或用纯化的包膜糖蛋白 gp120 免疫后会诱导产生细胞毒性抗体。
DOI：
10.1073/pnas.84.11.3797
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发表时间：
1987
影响因子：
11.1
通讯作者：
Fischinger,PJ
中科院分区：
文献类型：
--
作者：
Nara,PL;Robey,WG;Gonda,MA;Carter,SG;Fischinger,PJ
在能够感染人类免疫缺陷病毒分离物HTLV-IIIb的人类和黑猩猩中评估了抗体依赖性补体介导的细胞毒性（ACC）的存在，并在HTLV-IIIb的主要病毒糖蛋白（gp120）免疫后的山羊中检测了ACC的存在。在受感染的人类中，无论疾病状态如何，均未观察到介导ACC的抗体。即使是具有高滴度、广泛反应性和中和抗体的健康个体也没有ACC。相比之下，感染HTLV-IIIb病毒的黑猩猩不仅具有中和抗体，而且在ACC中具有广泛反应性的抗体，甚至可以对抗远亲的人类免疫缺陷病毒分离株，以及它们自己再分离的病毒。在山羊中，HTLV-IIIb的gp120诱导了高度类型特异性的反应，通过ACC和流式细胞荧光法检测感染的活H9细胞。动物抗htlv - iii gp120特异性血清对正常人细胞无影响。在动物实验模型中，ACC的诱导与中和抗体密切相关，但在人体中没有。在接种gp120的山羊和感染htlv - iii的黑猩猩中，ACC的存在代表了一种质的差异，这可能对寻求在人类中引发保护性免疫很重要。
The presence of antibody-dependent complement-mediated cytotoxicity (ACC) was assessed in humans and chimpanzees, which are capable of infection with human immunodeficiency virus isolate HTLV-IIIb, and examined in the goat after immunization with the major viral glycoprotein (gp120) of HTLV-IIIb. In infected humans no antibody mediating ACC was observed regardless of the status of disease. Even healthy individuals with high-titer, broadly reactive, neutralizing antibodies had no ACC. In contrast, chimpanzees infected with HTLV-IIIb, from whom virus could be isolated, not only had neutralizing antibody but also antibodies broadly reactive in ACC, even against distantly related human immunodeficiency virus isolates, as well as against their own reisolated virus. In the goat, the gp120 of HTLV-IIIb induced a highly type-specific response as measured by both ACC and flow cytofluorometry of live infected H9 cells. Normal human cells were not subject to ACC by animal anti-HTLV-III gp120-specific sera. Induction of ACC and neutralizing antibody were closely correlated in the animal experimental models but not in humans. The presence of ACC in gp120-inoculated goats and HTLV-III-infected chimpanzees represents a qualitative difference that may be important in the quest for the elicitation of a protective immunity in humans.