Changes in sugar composition and cellulose content during the secondary cell wall biogenesis in cotton fibers
Changes in sugar composition and cellulose content during the secondary cell wall biogenesis in cotton fibers
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DOI：
10.1007/s10570-009-9364-3
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发表时间：
2010-02-01
期刊：
影响因子：
5.7
通讯作者：
Cabrales, Luis
中科院分区：
文献类型：
--
作者：
Abidi, Noureddine;Hequet, Eric;Cabrales, Luis
棉花品种TX19和TX55(Gossypium hirsutom L.cv.)在温室中种植，并在不同发育阶段收获纤维。用高效液相色谱法测定纤维上的糖含量，用蒽酮法测定纤维中的纤维素含量。糖(蔗糖、葡萄糖、果糖和半乳糖醛酸)的百分比在纤维发育过程中表现出统计上的显著变化。随着次生细胞壁的发育，这些糖的百分比下降与纤维素含量的增加有关。必须指出的是，这些分析是在完整的纤维上进行的，没有进行细胞壁提取和纯化。
Two cotton cultivars TX19 and TX55 (Gossypium hirsutum L. cv.) were planted in the greenhouse and fibers were harvested at different stages of development. The percentage of sugars present on the fibers was determined by High Performance Liquid Chromatography and the cellulose content was determined using the anthrone method. The percentage of sugars (sucrose, glucose, fructose, and galacturonic acid) showed statistically significant changes during fiber development. The decrease in the percentages of these sugars as the secondary cell wall develops was associated with an increase in the cellulose content. It is important to point out that these analyses were done on intact fibers, no cell wall extractions and purifications were performed.